Search
引用和文献 (22)
Invitrogen™
NucRed™ Dead 647 ReadyProbes™ 试剂（ TO-PRO-3 碘化物）
NucRed Dead 647 ReadyProbes 试剂是一种细胞不可透过性染料，可在与 DNA 结合时发出明亮的远红外荧光。由于该试剂能够对质膜不完整的细胞进行染色，因此对于检测细胞活力以及动力学活细胞检测中的细胞毒性非常有用。NucRed Dead了解更多信息
| 货号 | 数量 |
|---|---|
| R37113 | 6瓶 |
货号 R37113
价格（CNY)
3,746.00
Each
数量:
6瓶
价格（CNY)
3,746.00
Each
NucRed Dead 647 ReadyProbes 试剂是一种细胞不可透过性染料，可在与 DNA 结合时发出明亮的远红外荧光。由于该试剂能够对质膜不完整的细胞进行染色，因此对于检测细胞活力以及动力学活细胞检测中的细胞毒性非常有用。NucRed Dead 647 试剂也适用于固定细胞制备和组织切片中的细胞核染色。
还可提供： 12.5X 浓度 (13.3 µM) 的 TO-PRO-3 碘化物水溶液（含 PowerLoad 增溶剂和 %1 DMSO）。
•与 DNA 结合后，发出非常明亮的远红外荧光
• 可快速染色受损、死亡细胞或固定细胞，无需清洗步骤
• 以方便的滴瓶包装的即用型液体配方
• 室温下稳定—可在您的范围或细胞培养区域内保持便利
查看其他用于细胞染色的 ReadyProbes 试剂
查看其他用于成像的细胞核染色剂
细胞成像应用
远红外荧光发射使其成为与蓝色、绿色和红色荧光基团（如 Hoechst、GFP/FITC 和 Texas Red）结合使用的理想选择。
使用建议
• NucRed Dead 647 ReadyProbes 试剂可以直接添加至完全培养基或缓冲溶液内的细胞中。
•在大多数情况下，2滴/mL 和15至30分钟孵育即可产生明亮的细胞核染色；然而为实现较佳的染色强度，可以添加更多或更少的液滴。
•在大多数情况下，如果在成像前不洗涤细胞，染色强度随时间的增加而增加。
• 可通过远红外滤光片检测，如 Cy5 滤光片或适用于 EVOS 成像系统的 Cy5 Light Cube。
还可提供： 12.5X 浓度 (13.3 µM) 的 TO-PRO-3 碘化物水溶液（含 PowerLoad 增溶剂和 %1 DMSO）。
•与 DNA 结合后，发出非常明亮的远红外荧光
• 可快速染色受损、死亡细胞或固定细胞，无需清洗步骤
• 以方便的滴瓶包装的即用型液体配方
• 室温下稳定—可在您的范围或细胞培养区域内保持便利
查看其他用于细胞染色的 ReadyProbes 试剂
查看其他用于成像的细胞核染色剂
细胞成像应用
远红外荧光发射使其成为与蓝色、绿色和红色荧光基团（如 Hoechst、GFP/FITC 和 Texas Red）结合使用的理想选择。
使用建议
• NucRed Dead 647 ReadyProbes 试剂可以直接添加至完全培养基或缓冲溶液内的细胞中。
•在大多数情况下，2滴/mL 和15至30分钟孵育即可产生明亮的细胞核染色；然而为实现较佳的染色强度，可以添加更多或更少的液滴。
•在大多数情况下，如果在成像前不洗涤细胞，染色强度随时间的增加而增加。
• 可通过远红外滤光片检测，如 Cy5 滤光片或适用于 EVOS 成像系统的 Cy5 Light Cube。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
形式液体
数量6瓶
子细胞定位细胞核/核仁
颜色远红外
发射661 nm
Excitation Wavelength Range642 nm
适用于（应用）细胞活力检测
适用于（设备）共聚焦显微镜, 荧光显微镜, 流式细胞仪
产品线NucRed、ReadyProbes
产品类型试剂名称
Unit SizeEach
内容与储存
6 x 2.5 mL 滴瓶
在≤25°C 下储存
在≤25°C 下储存
常见问题解答 (FAQ)
Can I use the ReadyProbes reagents for flow cytometry?
引用和文献 (22)
引用和文献
Abstract
Ammonia excretion in the Atlantic hagfish (Myxine glutinosa) and responses of an Rhc glycoprotein.
Journal:
PubMed ID:25715835
Hagfishes are the most ancient of the extant craniates, and demonstrate a high tolerance for a number of unfavorable environmental conditions including elevated ammonia. Proposed mechanisms of ammonia excretion in aquatic organisms include vesicular NH4+ transport and release by exocytosis in marine crabs, and passive NH3 diffusion, active NH4+ transport
ALS-associated FUS mutations result in compromised FUS alternative splicing and autoregulation.
Journal:
PubMed ID:24204307
The gene encoding a DNA/RNA binding protein FUS/TLS is frequently mutated in amyotrophic lateral sclerosis (ALS). Mutations commonly affect its carboxy-terminal nuclear localization signal, resulting in varying deficiencies of FUS nuclear localization and abnormal cytoplasmic accumulation. Increasing evidence suggests deficiencies in FUS nuclear function may contribute to neuron degeneration. Here
Hepatic DNA deposition drives drug-induced liver injury and inflammation in mice.
Journal:
PubMed ID:24824608
Drug-induced liver injury (DILI) is an important cause of acute liver failure, with limited therapeutic options. During DILI, oncotic necrosis with concomitant release and recognition of intracellular content amplifies liver inflammation and injury. Among these molecules, self-DNA has been widely shown to trigger inflammatory and autoimmune diseases; however, whether DNA
A map of gene expression in neutrophil-like cell lines.
Journal:BMC Genomics
PubMed ID:30068296
'Human neutrophils are central players in innate immunity, a major component of inflammatory responses, and a leading model for cell motility and chemotaxis. However, primary neutrophils are short-lived, limiting their experimental usefulness in the laboratory. Thus, human myeloid cell lines have been characterized for their ability to undergo neutrophil-like differentiation
Therapeutic Targeting of PTK7 is Cytotoxic in Atypical Teratoid Rhabdoid Tumors.
Journal:Mol Cancer Res
PubMed ID:28442586
'Novel discoveries involving the evaluation of potential therapeutics are based on newly identified molecular targets for atypical teratoid rhabdoid tumors (ATRT), which are the most common form of infantile brain tumors. Central nervous system ATRTs are rare, aggressive, and fast growing tumors of the brain and spinal cord and carry