碘化丙啶 Ready Flow™ 试剂

Invitrogen™

碘化丙啶 Ready Flow™ 试剂

碘化丙啶 Ready Flow™ 试剂是一种明亮、易于使用的核酸染料，用于在流式细胞分析中区分活细胞。该染料的激发/发射最大值为 535/617 nm。•可在室温下保持稳定• 便利的即用型配方—无需稀释了解更多信息

货号	数量
R37169	120 assays

货号 R37169

价格（CNY)

944.00

Ends: 31-Dec-2025

1,253.00

共减 309.00 (25%)

添加至购物车

120 assays

价格（CNY)

944.00

Ends: 31-Dec-2025

1,253.00

共减 309.00 (25%)

添加至购物车

碘化丙啶 Ready Flow™ 试剂是一种明亮、易于使用的核酸染料，用于在流式细胞分析中区分活细胞。该染料的激发/发射最大值为 535/617 nm。

•可在室温下保持稳定
• 便利的即用型配方—无需稀释、称重或者移取
• 无需洗涤步骤即可快速染色死细胞

碘化丙啶 Ready Flow 试剂是一种常用的死细胞染色剂，与 DNA 结合时会发出红色荧光。直接向 1 x 10⁶ 细胞中滴加 2 滴试剂，然后孵育、分析。

碘化丙啶是不透膜的，因此会从活细胞中洗脱出来。它通过嵌入到碱基之间以结合到 DNA，从而结合 DNA 和 RNA。碘化丙啶通常通过 488 nm 激光激发，但其他的激光（例如 532 和 561 nm 激光）也适合 PI 激发。

细胞渗透性细胞不可透过性

检测方法荧光

激发/发射535/617

数量120 assays

试剂类型核酸染色

子细胞定位细胞核/核仁

产品线Ready Flow™

产品类型荧光染色

Unit SizeEach

内容与储存

2 个滴瓶，储存（在室温下）

常见问题解答 (FAQ)

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

碘化丙啶 Ready Flow™ 试剂

常见问题解答 (FAQ)

I would like to label only 100 &micro;L of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

What is the concentration of dye/reagent in the Ready Flow products?

Can I use the ReadyProbes reagents for flow cytometry?

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?