ReadyProbes 2.5% Normal Goat Serum is a ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF)了解更多信息
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货号
数量
R37624
50 mL
货号 R37624
价格(CNY)
1,089.00
飞享价
Ends: 31-Dec-2025
1,465.00
共减 376.00 (26%)
Each
添加至购物车
数量:
50 mL
价格(CNY)
1,089.00
飞享价
Ends: 31-Dec-2025
1,465.00
共减 376.00 (26%)
Each
添加至购物车
ReadyProbes 2.5% Normal Goat Serum is a ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments, usually when a goat secondary antibody is used. Incubating cells/tissue with diluted serum from the same species as the host of the secondary antibody typically reduces non-specific binding. Simply cover the tissue section or cells with a few drops of ReadyProbes 2.5% Normal Goat Serum and incubate for 30–60 minutes at room temperature. Ready Probes 2.5% Normal Goat Serum can also be used to dilute primary and secondary antibodies.
Ready Probes 2.5% Normal Goat Serum is provided as a ready-to-use reagent with no further dilution or preparation required. The serum is collected from healthy adult animals and the heat-treated, centrifuged, and filtered. It is then checked for cross reactivity using an appropriate immunoassay. The serum is diluted to 2.5% (v/v) in buffer, with 0.08% sodium azide added as a preservative.
仅供科研使用。不可用于诊断程序。
规格
产品线ReadyProbes™
试剂类型封闭试剂
运输条件室温或湿冰
种属山羊
形式液体
产品类型山羊血清
数量50 mL
Unit SizeEach
内容与储存
以透明塑料滴瓶装浓度为即用型 (1X) 的 50 mL 溶液形式提供。
在 2–8°C 下储存。
按指示储存时,产品在接收后可以使用 6 个月。
常见问题解答 (FAQ)
Can I use ReadyProbes 2.5% Normal Goat Serum (1X) as an antibody diluent for both primary and secondary antibodies?
Yes, ReadyProbes 2.5% Normal Goat Serum (1X) can be used as an antibody diluent for both primary and secondary antibodies, except for HRP-conjugated antibodies. The sodium azide added as a preservative is an inhibitor of horseradish peroxidase.
When would I use goat, horse, or chicken serum for blocking versus FBS or BSA?
For blocking, you should use a serum of the same or closely related species of the secondary antibody being used. If using a secondary antibody that was raised in goat or sheep, use goat serum (Cat. No. R37624 or 31873) for blocking; if using a secondary antibody raised in donkey or horse, use horse serum (Cat. No. R37625 or 31874) for blocking; if using a secondary antibody raised in chicken, use chicken serum (Cat. No. R37626) for blocking. FBS and BSA are alternatives when a specific species of serum is not available.