Positope™ Control Protein
Positope™ Control Protein
Invitrogen™

Positope™ Control Protein

The Positope™ Control Protein is designed to be used as a positive control in western blotting for a variety of了解更多信息
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货号数量
R900505 μg
货号 R90050
价格(CNY)
3,530.00
Each
添加至购物车
数量:
5 μg
价格(CNY)
3,530.00
Each
添加至购物车
The Positope™ Control Protein is designed to be used as a positive control in western blotting for a variety of antibodies. The control protein is a 53 kDa highly purified (>95%) recombinant protein that contains seven epitope tags. Validated applications for the Positope Control Protein are Western Blot against the primary antibodies listed in the manual. By using Positope™ Protein as a positive control, you can assess the performance of your western blots, no matter which of the epitopes you are using.
Control Protein Attributes:
Applications: Validated applications for Positope™ Control Protein are Western blotting using the primary antibodies mentioned in the manual. Recommended loading amount for Western blotting: 100 ng per lane.
Protein Subtype: Tagged Protein Control
Product Size: Positope™ Control Protein is supplied as a 5 μg pack size.
For Research Use Only. Not for use in diagnostic procedures.
规格
产品线Positope™
蛋白质家族Other Proteins
蛋白质形式Recombinant
蛋白质子类型Tagged Protein Controls
数量5 μg
适用于(应用)Assay Standard
Unit SizeEach
内容与储存
Each vial contains 5 μg of Positope™ Control Protein at a concentration of 25 ng/μl in reducing SDS-PAGE sample buffer. Store the protein at +4°C. For long-term storage, store at -20°C. The protein is guaranteed stable for 6 months when stored properly.

常见问题解答 (FAQ)

在设计用于抗体制备的肽段时,我需要考虑什么?

我们建议疏水残基占序列中所有残基的50%或更少。确保每五个氨基酸至少有一个带电残基:这通常被认为可以增强肽段的溶解度。肽段(与折叠和掩盖疏水性氨基酸的多肽相比)太小而不能折叠,因此只有少数疏水性氨基酸可使它们不溶解。

其他指南包括:
•含有多个半胱氨酸、甲硫氨酸和色氨酸的肽段可能难以合成。
•一些序列在固相肽段合成或切割中存在问题,最好避免;这些序列包括天冬氨酸-脯氨酸(Asp-Pro)序列或在合成期间在其侧链上需要庞大保护基团的氨基酸片段。
•甘氨酸通常具有良好的抗原性,因为它只有一个氢侧链;这使其可以完全旋转。

请注意,我们提供定制抗体制备服务(https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production.html),包括使用我们专有的抗原分析仪和抗原制备工具(https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production/antigen-profiler-antigen-preparation.html)。

抗体制备的最合适的肽段长度是多少?

大多数多肽抗原的长度范围为12至16个残基,并且相对容易合成。9个残基或更短的肽段已经成为抗体制备的有效抗原,但是长于16个氨基酸的多肽可能含有多个表位并形成二级结构。超过18个残基的多肽开始出现更多的合成挑战。在合成您的多肽之前,我们建议您使用多肽序列进行BLASTP搜索。这是为了确保多肽与宿主动物中完全不相关的蛋白质中的序列不同源或相同。

请注意,我们提供定制抗体制备服务(https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production.html),包括使用我们专有的抗原分析仪和抗原制备工具(https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production/antigen-profiler-antigen-preparation.html)。

抗体对试剂盒包含什么?我可以处理多少样品,以及如何寻找按靶标提供的抗体对试剂盒列表?

抗体对试剂盒包含捕获抗体、检测抗体、重组标准品和HRP偶联物。每个套组都含有足够的试剂来处理四十个96孔板。可查看按靶标提供的抗体对试剂盒列表(https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html)。

试剂套组包含什么?我可以处理多少样品,以及如何检查试剂套组是否适用于我的靶标?

试剂套组包含捕获抗体、检测抗体、重组标准品、HRP偶联物、TMB底物和终止液。每个套组都含有足够的试剂来处理五个96孔板。试剂套组包含在ELISA试剂盒(https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html)的主列表中(通过“试剂套组”搜索)。

有多少种ELISA试剂盒?我应该如何按靶标查找ELISA试剂盒?

我们提供超过1000种不同的ELISA试剂盒,靶向> 800种不同的分子。您可以查看按靶标查找 ELISA 试剂盒页面(https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/elisa-kits/target-specific-elisa-kits.html)。