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ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.