There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.
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