Alexa Fluor™ 555 链霉亲和素偶联物

引用和文献 (10)

Invitrogen™

Alexa Fluor™ 555 链霉亲和素偶联物

Name: Alexa Fluor™ 555 链霉亲和素偶联物
SKU: S21381
Price: 4755.00 CNY

Alexa Fluor™ 555 链霉亲和素由生物素结合蛋白（链霉亲和素）与荧光标记物（Alexa Fluor™ 染料）共价结合而成了解更多信息

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货号	数量	形式
S21381	1 mg	实心
S32355	0.5 mL	液体

2 选项

货号 S21381

价格（CNY)

4,755.00

Each

添加至购物车

数量:

1 mg

形式:

实心

价格（CNY)

4,755.00

Each

添加至购物车

Alexa Fluor™ 555 链霉亲和素由生物素结合蛋白（链霉亲和素）与荧光标记物（Alexa Fluor™ 染料）共价结合而成。链霉亲和素与生物素结合的亲和力非常高，带链霉亲和素的偶联物通常和带生物素的偶联物一起使用，专门用于检测各种蛋白、蛋白模体、核酸或其他分子（例如与生物素偶联的一抗结合目标蛋白后，可以用荧光标记的链霉亲和素检测）。与此相似的检测策略在多种检测方法中使用，包括蛋白质免疫印迹、流式细胞分析、成像和显微检测、微孔板检测等。Alexa Fluor™ 染料链霉亲和素偶联物以 1 mg 冻干产品或 0.5 mL 体积的 2 mg/mL 溶液形式提供。

Alexa Fluor™ 555 链霉亲和素偶联物的重要特点：
•Alexa Fluor™ 555 链霉素亲和素偶联物的最大激发/发射波长分别为 ∼ (555/565)
• 光稳定性强荧光
• 在水溶液中的溶解度高
• 多种颜色可选
• 非常适合蛋白质免疫印迹、流式细胞分析、成像和显微镜检查、微孔板测定等应用

Alexa Fluor™ 染料的性质
Alexa Fluor™ 染料是专为提升成像和其他标记方案性能而开发的有机荧光染料，光稳定性和亮度更高，在水溶液中的溶解性更佳。这类染料提供多种颜色选择，是大多数成像应用的理想之选。

封闭内源性生物素
天然生成的生物素可干扰生物素-链霉亲和素检测方案。对于涉及固定或通透化细胞的实验，可使用我们的内源性生物素封闭试剂盒最大限度减少这种干扰。

仅限研究用途。不得用于人或动物的治疗或诊断。

相关链接：

在《Molecular Probes》手册中了解更多有关亲和素-生物素检测的信息

了解更多有关 Alexa Fluor™ 染料的信息

了解其他标记链霉亲和素偶联物

阅读亲和素和链霉亲和素偶联物 - 第7.6节

仅供科研使用。不可用于诊断程序。

规格

激发/发射555/565 nm

标签或染料Alexa Fluor 染料

产品类型链霉素亲和素偶联物(荧光)

数量1 mg

运输条件室温

偶联物Alexa Fluor 555

形式实心

产品线Alexa Fluor

Unit SizeEach

内容与储存

储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

引用和文献 (10)

引用和文献

Abstract

Seeing the trees in the forest: selective electroporation of adipocytes within adipose tissue.

Authors:Granneman JG, Li P, Lu Y, Tilak J

Journal:Am J Physiol Endocrinol Metab

PubMed ID:15126244

'Electroporation has been recently adapted for the transfer of macromolecules into cells of tissues in vivo. Although mature adipocytes constitute <20% of cells residing in adipose tissue, we hypothesized that fat cells might be susceptible to selective electrotransfer of plasmid DNA owing to their large size relative to other cells ... More

PKC putative phosphorylation site Ser235 is required for MIP/AQP0 translocation to the plasma membrane.

Authors:Golestaneh N, Fan J, Zelenka P, Chepelinsky AB,

Journal:Mol Vis

PubMed ID:18523655

'PURPOSE: To investigate the functional significance of MIP/AQP0 phosphorylation at serine(235). METHODS: MIP/AQP0 expression and cellular localization was studied in rat lens epithelia explants induced to differentiate by FGF-2. MIP wild type (WT) and MIP (S235A) mutant expression plasmids were constructed and transiently expressed in RK13 cells. Subcellular localization of ... More

Collagen XVI harbors an integrin alpha1 beta1 recognition site in its C-terminal domains.

Authors:Eble JA, Kassner A, Niland S, Mörgelin M, Grifka J, Grässel S

Journal:J Biol Chem

PubMed ID:16754661

'Collagen XVI is integrated tissue-dependently into distinct fibrillar aggregates, such as D-banded cartilage fibrils and fibrillin-1-containing microfibrils. In skin, the distribution of collagen XVI overlaps that of the collagen-binding integrins alpha1 beta1 and alpha2 beta1. Basal layer keratinocytes express integrin alpha2 beta1, whereas integrin alpha1 beta1 occurs in smooth muscle ... More

Diabetes is predicted by the beta cell level of autoantigen.

Authors:Byersdorfer CA, Schweitzer GG, Unanue ER

Journal:J Immunol

PubMed ID:16177075

'Two novel transgenic (Tg) strains were created expressing hen egg-white lysozyme (HEL) in a pancreas-specific fashion. RmHP.111 mice had levels of HEL per cell similar to that of the established ILK-3 strain, while RmHP.117 mice had 10-fold lower levels (50,000 molecules per cell). When bred to 3A9 TCR Tg mice, ... More

Metabolic and cellular plasticity in white adipose tissue II: role of peroxisome proliferator-activated receptor-alpha.

Authors:Li P, Zhu Z, Lu Y, Granneman JG

Journal:Am J Physiol Endocrinol Metab

PubMed ID:15941786

'Chronic activation of adipocyte beta-adrenergic receptors induces remodeling of white adipose tissue (WAT) that includes a transient inflammatory response followed by mitochondrial biogenesis, induction of fatty acid oxidation genes, and elevation of tissue oxidative metabolism. Gene profiling experiments of WAT during remodeling induced by the beta(3)-adrenergic receptor agonist CL-316,243 (CL) ... More

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