Discrepancy in measuring CD4 expression on T-lymphocytes using fluorescein conjugates in comparison with unimolar CD4-phycoerythrin conjugates.
Authors:Wang L, Abbasi F, Gaigalas AK, Hoffman RA, Flagler D, Marti GE,
Journal:Cytometry B Clin Cytom
PubMed ID:17474131
'BACKGROUND: Numerous methods for quantitative fluorescence calibration (QFC) have been developed to quantify receptor expression on lymphocytes. However, the results from the use of these different QFC methods vary considerably in the literature. To better identify the causes of these discrepancies, we measured CD4 expression using FITC and phycoerythrin (PE) ... More