SNARF™-5F 5-(和-6)-羧酸
SNARF™-5F 5-(和-6)-羧酸
Invitrogen™

SNARF™-5F 5-(和-6)-羧酸

不可渗透细胞的比率式 pH 值指示剂 SNARF™-5F 5-(和-6)-羧酸(pKa 为 ∼7.2)在酸性和碱性条件下分别显示出从黄橙色到深红色荧光的显著 pH了解更多信息
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货号数量
S23922
又称 S-23922
1 mg
货号 S23922
又称 S-23922
价格(CNY)
4,538.00
Online Exclusive
Ends: 31-Dec-2026
6,216.00
共减 1,678.00 (27%)
Each
添加至购物车
数量:
1 mg
价格(CNY)
4,538.00
Online Exclusive
Ends: 31-Dec-2026
6,216.00
共减 1,678.00 (27%)
Each
添加至购物车
不可渗透细胞的比率式 pH 值指示剂 SNARF™-5F 5-(和-6)-羧酸(pKa 为 ∼7.2)在酸性和碱性条件下分别显示出从黄橙色到深红色荧光的显著 pH 值依赖性发射波长变化。这种 pH 值依赖性使得可利用染料在两种发射波长(通常为 580 nm 和 640 nm)处的荧光强度比定量测定 pH 值。

了解有关离子指示剂(包括钙、钾、pH 值和膜电位指示剂)的更多信息›
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
指示器pH 值指示剂
数量1 mg
运输条件室温
适用于(设备)荧光显微镜, 流式细胞仪, 微孔板读数仪
产品线SNARF
产品类型pH 值指示剂
Unit SizeEach
内容与储存
室温避光储存。

引用和文献 (6)

引用和文献
Abstract
Characterization of dual-wavelength seminaphthofluorescein and seminapthorhodafluor dyes for pH sensing under high hydrostatic pressures.
Authors:Salerno M, Ajimo JJ, Dudley JA, Binzel K, Urayama P
Journal:Anal Biochem
PubMed ID:17274941
'Hydrostatic pressure is an important physical parameter in biology, with pressures in the few-hundred-atm range having significant effects on cellular morphology, metabolism, and viability. To ensure valid results when studying pressure effects using fluorescence spectroscopy and imaging methods, metabolic probes need to be characterized for high-pressure use. Of interest is ... More
Fluorescent indicators for intracellular pH.
Authors:Han J, Burgess K,
Journal:Chem Rev
PubMed ID:19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More
Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.
Authors:Liu J, Diwu Z, Leung WY
Journal:Bioorg Med Chem Lett
PubMed ID:11677123
Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission ... More
Using LysoSensor Yellow/Blue DND-160 to sense acidic pH under high hydrostatic pressures.
Authors:DePedro HM, Urayama P,
Journal:Anal Biochem
PubMed ID:18976626
LysoSensor Yellow/Blue DND-160, a dual-wavelength fluorophore commonly used for sensing pH in acidic organelles, possesses solvatochromic behavior believed to originate from an intramolecular charge transfer (ICT). Given this, we investigated whether DND-160 can be used for acidic pH sensing under hydrostatic pressures up to 510 atm, a range suitable for ... More
The chloride transporter Na(+)-K(+)-Cl- cotransporter isoform-1 contributes to intracellular chloride increases after in vitro ischemia.
Authors:Pond BB, Berglund K, Kuner T, Feng G, Augustine GJ, Schwartz-Bloom RD
Journal:J Neurosci
PubMed ID:16452663
Ischemic episodes in the CNS cause significant disturbances in neuronal ionic homeostasis. To directly measure changes in intracellular Cl- concentration ([Cl-]i) during and after ischemia, we used Clomeleon, a novel ratiometric optical indicator for Cl-. Hippocampal slices from adult transgenic mice expressing Clomeleon in hippocampal neurons were subjected to 8 ... More