引用和文献 (3)

Invitrogen™

SAIVI™ 快速抗体标记试剂盒，Alexa Fluor™ 750

货号	数量
S30046	1 个试剂盒

货号 S30046

价格（CNY)

13,468.00

1 个试剂盒

价格（CNY)

13,468.00

Alexa Fluor™ 750 SAIVI™ 抗体标记试剂盒提供了一种标记抗体的便利方法，在 6 倍抗体浓度范围内具有适用于体内成像应用的较优标记度 (DOL; ∼2)，无需调整反应体积、染料浓度或抗体浓度。使用该程序，较佳标记抗体就可以直接用于需要不含叠氮化物试剂的应用，例如活细胞成像或直接注射至动物。

仅供科研使用。不可用于诊断程序。

颜色红外的

检测方法荧光

激发/发射749/775

标签类型Alexa Fluor 染料

标记规模0.5 至 3 mg

产品线Alexa Fluor、SAIVI

产品类型抗体标记试剂盒

数量1 个试剂盒

运输条件室温

标记目标抗体

标签或染料Alexa Fluor 750

Unit SizeEach

内容与储存

在冷藏冰箱（2°C 至 8°C）中避光储存。

常见问题解答 (FAQ)

我该注射多少量的偶联抗体来进行肿瘤成像？

推荐的起始剂量为50 µg。您需要根据实验模型来确定最佳剂量。

我应该采用哪种规格的针头？

我们建议使用具有固定（不可移动）针头、28-32管径的结核菌素和胰岛素注射器（0.3或1.0 mL容量）。

能够注入小鼠体内的最佳和最大试剂体积是多少？

注射试剂的体积根据给药途径而异。下面是25克动物的一般指导用量：

静脉注射（IV）50–125 µL（推荐体积）- 200 µL（最大体积）；腹腔注射（IP）500 µL（推荐体积）- 2 mL（最大体积）；皮下注射（SC）100–250 µL（推荐体积）- 1 mL（最大体积）。

哪种类型的小鼠最适于体内成像？

由于毛皮会引起光散射，所以推荐采用无毛小鼠（如无胸腺裸鼠（nu/nu））进行体内成像。如果无法使用裸鼠，覆盖成像区域的毛发应该用剃毛机或化学脱毛剂（如Nair脱毛剂）去除。

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all SAIVI™ 快速抗体标记试剂盒，Alexa Fluor™ 750 FAQs

引用和文献 (3)

引用和文献

Abstract

Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.

Authors:Lee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,

Journal:Clin Cancer Res

PubMed ID:18559604

'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More

Blocking ephrinB2 with highly specific antibodies inhibits angiogenesis, lymphangiogenesis, and tumor growth.

Authors:Abéngozar MA, de Frutos S, Ferreiro S, Soriano J, Perez-Martinez M, Olmeda D, Marenchino M, Cañamero M, Ortega S, Megias D, Rodriguez A, Martínez-Torrecuadrada JL,

Journal:Blood

PubMed ID:22446484

Membrane-anchored ephrinB2 and its receptor EphB4 are involved in the formation of blood and lymphatic vessels in normal and pathologic conditions. Eph/ephrin activation requires cell-cell interactions and leads to bidirectional signaling pathways in both ligand- and receptor-expressing cells. To investigate the functional consequences of blocking ephrinB2 activity, 2 highly specific ... More

A leukocyte-mimetic magnetic resonance imaging contrast agent homes rapidly to activated endothelium and tracks with atherosclerotic lesion macrophage content.

Authors:McAteer MA, Mankia K, Ruparelia N, Jefferson A, Nugent HB, Stork LA, Channon KM, Schneider JE, Choudhury RP,

Journal:Arterioscler Thromb Vasc Biol

PubMed ID:22499989

Endothelial cell activation is an important mediator of monocyte recruitment to sites of vascular inflammation. We hypothesized that high-affinity dual-ligand microparticles of iron oxide (MPIO), targeted to P-selectin and vascular cell adhesion molecule-1 (PV-MPIO), would identify activated endothelial cells during atherosclerosis progression. In vivo magnetic resonance imaging in apolipoprotein E-deficient ... More