所有SYBR染料都具有相似的光谱性质,但化学组成不同。所有SYBR染料都与dsDNA、ssDNA和RNA结合,但敏感性不同。SYBRSafe DNA凝胶染料是专门研发的溴化乙锭安全替代品。SYBR Green I是dsDNA的超灵敏染料,而SYBR Green II是RNA和ssDNA的高灵敏性染料。所有SYBR染料都适用于Safe Imager蓝光透射仪激发观察。
各种SYBR染料有何区别?
所有SYBR染料都与dsDNA、ssDNA和RNA结合,但敏感性不同。SYBR Green I用于dsDNA和ssDNA染色。SYBR Green II可以对dsDNA和ssDNA进行染色,但对RNA的敏感性更好。SYBR Gold
是在SYBR Green I和II之后开发的,是最敏感的荧光凝胶染色剂。SYBR Safe DNA凝胶染色剂是一种用于蓝光系统的致突变性更低的配方。它不像SYBR Green I和II那么敏感,但可与溴化乙锭相比。
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SPACRCAN, a novel human interphotoreceptor matrix hyaluronan-binding proteoglycan synthesized by photoreceptors and pinealocytes.
Authors:Acharya S, Foletta VC, Lee JW, Rayborn ME, Rodriguez IR, Young WS, Hollyfield JG
Journal:J Biol Chem
PubMed ID:10702256
The interphotoreceptor matrix is a unique extracellular complex occupying the interface between photoreceptors and the retinal pigment epithelium in the fundus of the eye. Because of the putative supportive role in photoreceptor maintenance, it is likely that constituent molecules play key roles in photoreceptor function and may be targets for ... More
Lateral-flow and up-converting phosphor reporters to detect single-stranded nucleic acids in a sandwich-hybridization assay.
Authors:Corstjens PL, Zuiderwijk M, Nilsson M, Feindt H, Sam Niedbala R, Tanke HJ
Journal:Anal Biochem
PubMed ID:12531205
'Up-converting Phosphor Technology (UPT) particles were used as reporters in lateral-flow (LF) assays to detect single-stranded nucleic acids. The 400-nm phosphor particles exhibit strong visible luminescence upon excitation with infrared (IR) light resulting in the total absence of background autofluorescence from other biological compounds. A sandwich-type hybridization assay was applied ... More
Naturally occurring mutations in human mitochondrial pre-tRNASer(UCN) can affect the transfer ribonuclease Z cleavage site, processing kinetics, and substrate secondary structure.
Authors:Yan H, Zareen N, Levinger L
Journal:J Biol Chem
PubMed ID:16361254
'tRNAs are transcribed as precursors with a 5'' end leader and a 3'' end trailer. The 5'' end leader is processed by RNase P, and in most organisms in all three kingdoms, transfer ribonuclease (tRNase) Z can endonucleolytically remove the 3'' end trailer. Long ((L)) and short ((S)) forms of ... More
Protein-free parallel triple-stranded DNA complex formation.
'A 14 nt DNA sequence 5''-AGAATGTGGCAAAG-3'' from the zinc finger repeat of the human KRAB zinc finger protein gene ZNF91 bearing the intercalator 2-methoxy,6-chloro,9-amino acridine (Acr) attached to the sugar-phosphate backbone in various positions has been shown to form a specific triple helix (triplex) with a 16 bp hairpin (intramolecular) ... More