SYBR™ Green II RNA 凝胶染料,10,000 X 浓缩于 DMSO
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SYBR™ Green II RNA 凝胶染料,10,000 X 浓缩于 DMSO
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SYBR™ Green II RNA 凝胶染料,10,000 X 浓缩于 DMSO

SYBR Green II RNA 凝胶染料是一种灵敏的核酸凝胶染料,与 RNA 结合时发出明亮的荧光且在凝胶中背景低,这使其成为与甲醛/琼脂糖或聚丙烯酰胺凝胶(使用激光扫描仪或标准紫外线透射仪)一起使用的理想选择。有了解更多信息
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货号数量
S758620 x 50 μL
S7564500 μL
S75681 mL
货号 S7586
价格(CNY)
7,049.00
飞享价
Ends: 31-Dec-2025
12,073.00
共减 5,024.00 (42%)
Each
添加至购物车
数量:
20 x 50 μL
请求批量或定制报价
价格(CNY)
7,049.00
飞享价
Ends: 31-Dec-2025
12,073.00
共减 5,024.00 (42%)
Each
添加至购物车
SYBR Green II RNA 凝胶染料是一种灵敏的核酸凝胶染料,与 RNA 结合时发出明亮的荧光且在凝胶中背景低,这使其成为与甲醛/琼脂糖或聚丙烯酰胺凝胶(使用激光扫描仪或标准紫外线透射仪)一起使用的理想选择。有 500 μL 单位规格 (S-7564) 和 1 mL 单位规格 (S-7568) 可选。
仅供科研使用。不可用于诊断程序。
规格
检测定位凝胶内检测
检测方法荧光
产品类型RNA 凝胶染料
数量20 x 50 μL
运输条件室温
靶标分子RNA
标签或染料SYBR Green II
Unit SizeEach
内容与储存
• 以 DMSO 配制的 10,000X 浓缩液形式提供

在 -20°C 的干燥器内避光储存。

常见问题解答 (FAQ)

SYBR染料的pH范围是多少?

SYBR染料仅在很窄的pH范围内有效,约为pH 7-8。超出此范围,荧光信号将迅速消失。

推荐凝胶成像系统使用什么滤光片?

请点击此处(https://www.thermofisher.com/cn/zh/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html)并选择“滤光片推荐”,查看适用于SYBR Safe DNA凝胶染料的推荐滤光片。请注意,SYBR Safe DNA凝胶染料的激发和发射光谱与SYBR Green I、SYBR Green II、SYBR Gold 染料,还有荧光素(FITC)极为相似。因此,亦可使用适用于这些染料的滤光片。若使用Safe Imager蓝光透射仪,则无需使用相机滤光片;仪器内提供的琥珀色滤光片即具有该功能。

我能否在我的相机上使用溴化乙锭滤光片对SYBR染料进行成像吗?

不推荐。大部分深琥珀/橙色溴化乙锭滤光片在550nm附近有临界值。SYBR Green染料在520nm处有发射光,所以使用这种滤光片会过滤掉您所需要的数据。

SYBR Safe DNA凝胶染料与SYBR Green I染料相同吗?

所有SYBR染料都具有相似的光谱性质,但化学组成不同。所有SYBR染料都与dsDNA、ssDNA和RNA结合,但敏感性不同。SYBRSafe DNA凝胶染料是专门研发的溴化乙锭安全替代品。SYBR Green I是dsDNA的超灵敏染料,而SYBR Green II是RNA和ssDNA的高灵敏性染料。所有SYBR染料都适用于Safe Imager蓝光透射仪激发观察。

各种SYBR染料有何区别?

所有SYBR染料都与dsDNA、ssDNA和RNA结合,但敏感性不同。SYBR Green I用于dsDNA和ssDNA染色。SYBR Green II可以对dsDNA和ssDNA进行染色,但对RNA的敏感性更好。SYBR Gold 是在SYBR Green I和II之后开发的,是最敏感的荧光凝胶染色剂。SYBR Safe DNA凝胶染色剂是一种用于蓝光系统的致突变性更低的配方。它不像SYBR Green I和II那么敏感,但可与溴化乙锭相比。 < / p >

引用和文献 (26)

引用和文献
Abstract
Authors:
Journal:
PubMed ID:8660526
SPACRCAN, a novel human interphotoreceptor matrix hyaluronan-binding proteoglycan synthesized by photoreceptors and pinealocytes.
Authors:Acharya S, Foletta VC, Lee JW, Rayborn ME, Rodriguez IR, Young WS, Hollyfield JG
Journal:J Biol Chem
PubMed ID:10702256
The interphotoreceptor matrix is a unique extracellular complex occupying the interface between photoreceptors and the retinal pigment epithelium in the fundus of the eye. Because of the putative supportive role in photoreceptor maintenance, it is likely that constituent molecules play key roles in photoreceptor function and may be targets for ... More
Lateral-flow and up-converting phosphor reporters to detect single-stranded nucleic acids in a sandwich-hybridization assay.
Authors:Corstjens PL, Zuiderwijk M, Nilsson M, Feindt H, Sam Niedbala R, Tanke HJ
Journal:Anal Biochem
PubMed ID:12531205
'Up-converting Phosphor Technology (UPT) particles were used as reporters in lateral-flow (LF) assays to detect single-stranded nucleic acids. The 400-nm phosphor particles exhibit strong visible luminescence upon excitation with infrared (IR) light resulting in the total absence of background autofluorescence from other biological compounds. A sandwich-type hybridization assay was applied ... More
Naturally occurring mutations in human mitochondrial pre-tRNASer(UCN) can affect the transfer ribonuclease Z cleavage site, processing kinetics, and substrate secondary structure.
Authors:Yan H, Zareen N, Levinger L
Journal:J Biol Chem
PubMed ID:16361254
'tRNAs are transcribed as precursors with a 5'' end leader and a 3'' end trailer. The 5'' end leader is processed by RNase P, and in most organisms in all three kingdoms, transfer ribonuclease (tRNase) Z can endonucleolytically remove the 3'' end trailer. Long ((L)) and short ((S)) forms of ... More
Protein-free parallel triple-stranded DNA complex formation.
Authors:Shchyolkina AK, Timofeev EN, Lysov YP, Florentiev VL, Jovin TM, Arndt-Jovin DJ
Journal:Nucleic Acids Res
PubMed ID:11160932
'A 14 nt DNA sequence 5''-AGAATGTGGCAAAG-3'' from the zinc finger repeat of the human KRAB zinc finger protein gene ZNF91 bearing the intercalator 2-methoxy,6-chloro,9-amino acridine (Acr) attached to the sugar-phosphate backbone in various positions has been shown to form a specific triple helix (triplex) with a 16 bp hairpin (intramolecular) ... More