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引用和文献 (28)
Invitrogen™
链霉亲和素-碱性磷酸酶偶联物
Thermo Scientific 链霉素亲和素-碱性磷酸酶偶联物包括纯化形式的链霉素亲和素，与碱性磷酸酶结合，用于基于底物的检测。链霉素亲和素是一种起初从阿维丁链霉菌中分离出的生物素结合蛋白。与亲和素相比，链霉素亲和素没有碳水化合物且弱酸性 pI 为 5。Thermo Scientific了解更多信息
| 货号 | 数量 |
|---|---|
| S921 | 0.5 mL |
货号 S921
价格（CNY)
6,814.00
Each
数量:
0.5 mL
价格（CNY)
6,814.00
Each
Thermo Scientific 链霉素亲和素-碱性磷酸酶偶联物包括纯化形式的链霉素亲和素，与碱性磷酸酶结合，用于基于底物的检测。
链霉素亲和素是一种起初从阿维丁链霉菌中分离出的生物素结合蛋白。与亲和素相比，链霉素亲和素没有碳水化合物且弱酸性 pI 为 5。Thermo Scientific 链霉素亲和素产品使用重组形式的链霉素亲和素，其质量为 53,000 道尔顿，pI 接近中性。链霉素亲和素是四聚体蛋白，各亚单元结合一分子生物素，亲和性与亲和素相似。盐酸胍可以将亲和链霉素亲和素解离为亚单元，但链霉素亲和素对解离耐受性更强。
链霉素亲和素-碱性磷酸酶偶联物能够在多种标准测定方法（包括 Western 印迹、ELISA、免疫组织化学技术和荧光成像）中检测生物素化抗体和其他探针。
仅供科研使用。不可用于诊断程序。
规格
最大浓度2 mg/mL
标签或染料AP(碱性磷酸酶)
产品类型链霉素亲和素偶联物(荧光)
数量0.5 mL
运输条件湿冰
偶联物AP
形式液体
Unit SizeEach
内容与储存
储存在冰箱 (2–8°C) 中。
引用和文献 (28)
引用和文献
Abstract
Enumeration of antigen-specific IgE responses at the single-cell level by an ELISA plaque assay.
Journal:J Immunol Methods
PubMed ID:1703186
In vitro elicitation and enumeration of antigen-specific IgE-secreting cells in antigen-primed murine lymphocyte cultures can be reproducibly and accurately quantitated. Rat anti-mouse IgE monoclonal antibodies (Mab anti-epsilon) were developed. Antigen-specific IgE-secreting cells can be detected on antigen or MAb anti-epsilon coated nitrocellulose discs pasted to 24-well culture plates. This IgE
Detection of low copy numbers of HPV DNA by fluorescent in situ hybridization combined with confocal microscopy as an alternative to in situ polymerase chain reaction.
Journal:J Virol Methods
PubMed ID:9672129
'In genital lesions infected by human papillomavirus (HPV), histological criteria and HPV DNA typing are of prognostic value. Therefore, non-radioactive methods such as in situ hybridization are used extensively since they preserve the histological organization of the tissue, and allow the detection and characterization of HPV DNA. However, the sensitivity
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Journal:Anal Biochem
PubMed ID:12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by
Binding of monoclonal antibody AA4 to gangliosides on rat basophilic leukemia cells produces changes similar to those seen with Fc epsilon receptor activation.
Journal:J Cell Biol
PubMed ID:1370498
'The mAb AA4 binds to novel derivatives of the ganglioside Gd1b on rat basophilic leukemia (RBL-2H3) cells. Some of the gangliosides are located close to the high affinity IgE receptor (Fc epsilon RI), and binding of mAb AA4 inhibits Fc epsilon RI-mediated histamine release. In the present study, mAb AA4
Ubiquitination and proteasomal activity is required for transport of the EGF receptor to inner membranes of multivesicular bodies.
Journal:J Cell Biol
PubMed ID:11864992
'EGF, but not TGF alpha, efficiently induces degradation of the EGF receptor (EGFR). We show that EGFR was initially polyubiquitinated to the same extent upon incubation with EGF and TGF alpha, whereas the ubiquitination was more sustained by incubation with EGF than with TGF alpha. Consistently, the ubiquitin ligase c-Cbl