NoLimits 750 bp DNA 片段
NoLimits 750 bp DNA 片段
Thermo Scientific™

NoLimits 750 bp DNA 片段

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Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other了解更多信息
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货号数量
SM147110 μg
货号 SM1471
价格(CNY)
1,108.00
10 µg
添加至购物车
数量:
10 μg
请求批量或定制报价
价格(CNY)
1,108.00
10 µg
添加至购物车
Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.
仅供科研使用。不可用于诊断程序。
规格
凝胶兼容性琼脂糖凝胶
环保功能绿色可持续包装
产品线NoLimits
产品类型DNA 片段
数量10 μg
即用型
凝胶类型琼脂糖
大小范围10 bp 至 20000 bp
Unit Size10 µg

常见问题解答 (FAQ)

Are the Thermo Scientific DNA ladders compatible with GelRed and GelGreen gel stains?

The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.

Which Thermo Scientific DNA ladders are optimal for fast electrophoresis separation?

For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).

What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.