来自人血清的转铁蛋白,荧光素偶联物
来自人血清的转铁蛋白,荧光素偶联物
Invitrogen™

来自人血清的转铁蛋白,荧光素偶联物

转铁蛋白是一种单体血清糖蛋白(∼80,000 道尔顿),其通过受体介导的内吞作用结合到脊椎动物细胞表面的特定受体上,并递送多达 2 个 Fe3+ 原子 — 标记的了解更多信息
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货号数量
T28715 mg
货号 T2871
价格(CNY)
6,726.00
5 mg
添加至购物车
数量:
5 mg
价格(CNY)
6,726.00
5 mg
添加至购物车
转铁蛋白是一种单体血清糖蛋白(∼80,000 道尔顿),其通过受体介导的内吞作用结合到脊椎动物细胞表面的特定受体上,并递送多达 2 个 Fe3+ 原子 — 标记的 LDL 复合物是研究该现象的有用工具。携带铁的转铁蛋白进入核内体后,酸性环境即可促使铁离子从转铁蛋白–受体复合物中解离。铁释放后,脱铁转铁蛋白被回收至细胞膜中,其从受体中释放出来以结合更多的铁。因此,荧光转铁蛋白偶联物可以与荧光 LDL 配合使用,以区分溶菌体定向途径和内涵体回收通路。

这些实验通常是通过向培养细胞中加入荧光标记的转铁蛋白并通过显微镜进行分析。我们提供生物素化的转铁蛋白偶联物及超过 10 种荧光形式。

转铁蛋白规格:

标记 (Ex/Em):荧光素 (495/518)

含量:15 mg 固体(含 5 mg 转铁蛋白偶联物)

标记的转铁蛋白的主要应用
标记的转铁蛋白的其中一些应用包括:
•使用 FRET 对转铁蛋白受体动力学进行成像
• 通过共聚焦激光扫描显微镜检查观察活细胞中的受体转运
• 研究内涵体酸化期间发生的事件
• 测量哺乳动物和寄生虫中的转铁蛋白受体结合亲和力

仅供研究使用。不得用于任何动物或人类的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型经典染料
激发/发射495/518
形式实心
蛋白质家族转铁蛋白
数量5 mg
运输条件室温
产品类型转铁蛋白
pH7.2
Unit Size5 mg
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (103)

引用和文献
Abstract
Characterization of Endophilin B1b, a brain-specific membrane-associated lysophosphatidic acid acyl transferase with properties distinct from endophilin A1.
Authors:Modregger J, Schmidt AA, Ritter B, Huttner WB, Plomann M
Journal:J Biol Chem
PubMed ID:12456676
We have characterized mammalian endophilin B1, a novel member of the endophilins and a representative of their B subgroup. The endophilins B show the same domain organization as the endophilins A, which contain an N-terminal domain responsible for lipid binding and lysophosphatidic acid acyl transferase activity, a central coiled-coil domain ... More
Interactions between Piccolo and the actin/dynamin-binding protein Abp1 link vesicle endocytosis to presynaptic active zones.
Authors:Fenster SD, Kessels MM, Qualmann B, Chung WJ, Nash J, Gundelfinger ED, Garner CC
Journal:J Biol Chem
PubMed ID:12654920
'Piccolo is a high molecular weight multi-domain protein shown to be a structural component of the presynaptic CAZ (cytoskeletal matrix assembled at active zones). These features indicate that Piccolo may act to scaffold proteins involved in synaptic vesicle endo- and exocytosis near their site of action. To test this hypothesis, ... More
Receptor-mediated endocytosis of transferrin and the uptake of fe in K562 cells: identification of a nonlysosomal acidic compartment.
Authors:van Renswoude J, Bridges KR, Harford JB, Klausner RD
Journal:Proc Natl Acad Sci U S A
PubMed ID:6292894
'At physiological temperature, the Fe-carrier transferrin is taken up by K562 human erythroleukemia cells through receptor-mediated endocytosis. Both ligand (now minus Fe) and receptor recycle back to the cell surface where the receptor is rapidly reutilized. After endocytosis, transferrin becomes transiently lodged within an acidic compartment inside the cell, as ... More
Defective acidification of intracellular organelles in cystic fibrosis.
Authors:Barasch J, Kiss B, Prince A, Saiman L, Gruenert D, al-Awqati Q
Journal:Nature
PubMed ID:1712081
'The phenotype of cystic fibrosis (CF) includes abnormalities in transepithelial transport of Cl- (refs 1-5), decreased sialylation and increased sulphation and fucosylation of glycoproteins, and lung colonization with Pseudomonas. It is not apparent how these abnormalities are interrelated, nor how they result from loss of function of the CF gene-encoded ... More
Imaging of receptor trafficking by using alpha-bungarotoxin-binding-site-tagged receptors.
Authors:Sekine-Aizawa Y, Huganir RL,
Journal:Proc Natl Acad Sci U S A
PubMed ID:15563595
'alpha-Amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA) receptors mediate excitatory synaptic transmission and are dynamically regulated during synaptic plasticity in the CNS. The membrane trafficking of AMPA receptors to synapses is critical for the regulation of the efficacy of excitatory synaptic transmission. Direct imaging of AMPA receptors in various cell compartments is important to dissecting ... More