Can fluorescent protein-expressing cells be fixed?
Yes, fluorescent protein-expressing cells can be fixed using 4% paraformaldehyde in PBS for 10 min followed by one quick PBS rinse and 3 x 5 min washes with 1 mL PBS.
Are the fluorescent proteins offered by Thermo Fisher Scientific (EmGFP, YFP, CFP, BFP and Cycle 3 GFP) humanized?
Yes, all of the fluorescent proteins offered by (EmGFP, YFP, CFP, BFP and Cycle 3 GFP) have been humanized for optimal mammalian expression.