用于进行流式细胞分析的含 Mitotracker™ Red & Annexin V Alexa Fluor™ 488 的线粒体膜电位细胞凋亡试剂盒
用于进行流式细胞分析的含 Mitotracker™ Red & Annexin V Alexa Fluor™ 488 的线粒体膜电位细胞凋亡试剂盒
Invitrogen™

用于进行流式细胞分析的含 Mitotracker™ Red & Annexin V Alexa Fluor™ 488 的线粒体膜电位细胞凋亡试剂盒

这种流式细胞分析产品提供了一种快速且便利的用于检测细胞凋亡的两个特征-磷脂酰丝氨酸外化和线粒体膜电位变化的检测试剂。使用所提供的结合缓冲液中的 Alexa Fluor™ 488 Annexin V 和 MitoTracker™ Red了解更多信息
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货号数量
V351161 kit
货号 V35116
价格（CNY)
5,523.00
飞享价
Ends: 31-Dec-2025
7,339.00
共减 1,816.00 (25%)
1 kit
添加至购物车
数量:
1 kit
价格（CNY)
5,523.00
飞享价
Ends: 31-Dec-2025
7,339.00
共减 1,816.00 (25%)
1 kit
添加至购物车
这种流式细胞分析产品提供了一种快速且便利的用于检测细胞凋亡的两个特征-磷脂酰丝氨酸外化和线粒体膜电位变化的检测试剂。使用所提供的结合缓冲液中的 Alexa Fluor™ 488 Annexin V 和 MitoTracker™ Red CMXRos 染料对一个细胞群染色后，活细胞显示出的绿色荧光很少而红色荧光较强，而凋亡细胞显示出强烈的绿色荧光且红色荧光有所降低。这些细胞群可以很容易地通过流式细胞仪加以区分，氩离子激光器激发的 488 nm 光谱线可用于激发这两种染料。

查看用于流式细胞分析的所有细胞凋亡试验的选择指南。
仅供科研使用。不可用于诊断程序。
规格
激发/发射Mitotracker™ Red 579⁄599、Alexa Fluor™ 488：499⁄521
流式细胞仪激光线路488
适用于（应用）流式细胞分析
适用于（设备）荧光显微镜、流式细胞仪
反应次数50
产品线Alexa Fluor™、MitoTracker™
产品类型线粒体膜细胞凋亡试剂盒
数量1 kit
运输条件湿冰
偶联物Alexa Fluor™ 488、MitoTracker™ Red
产品规格管、玻片
Unit Size1 kit
内容与储存
含 1 小瓶 Annexin Alexa Fluor™ 488 偶联物 (250 µL)、3 小瓶 MitoTracker™ Red（50 µg/小瓶）、1 瓶膜联蛋白结合缓冲液（5X 溶液，15 mL）和 1 小瓶 DMSO (100 µL)。

避光储存在冷藏冰箱 (2–8°C) 中。

常见问题解答 (FAQ)

What are the fluorescence excitation/emission maxima for the dyes contained in the Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker Red & Alexa Fluor 488 annexin V, for flow cytometry

Alexa Fluor 488 annexin V: 499/521 nm
MitoTracker Red: 579/599 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.