pAc5.1/V5-His A、B & C 载体
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引用和文献 (7)
Invitrogen™

pAc5.1/V5-His A、B & C 载体

pAC5.1/V5-His vectors are designed for use in Drosophila cells to achieve high-level transient expression of recombinant proteins. The vector offers了解更多信息
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货号数量
V41102020 μg
货号 V411020
价格（CNY)
13,678.00
20 µg
添加至购物车
数量:
20 μg
价格（CNY)
13,678.00
20 µg
添加至购物车
pAC5.1/V5-His vectors are designed for use in Drosophila cells to achieve high-level transient expression of recombinant proteins. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His vectors also offer the following features:

• Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
仅供科研使用。不可用于诊断程序。
规格
产品规格冻干
产品类型果蝇表达载体
数量20 μg
载体pAC
克隆方法限制性内切酶/MCS
促进剂pAC5
蛋白标记His 标签 (6x)、V5 抗原决定簇标签, V5 Epitope Tag
Unit Size20 µg
内容与储存
含有各 20 µg 冻干 pAc5.1/V5-His A、B、C 和 pAc5.1/V5-His/LacZ。
在 -20°C 下储存。妥善储存时，可保证所有组分稳定储存 6 个月。

引用和文献 (7)

引用和文献
Abstract
Gata factor translation is the final downstream step in the amino acid/tor-mediated vitellogenin gene expression in the anautogenous mosquito aedes aegypti.
Authors:Park JH, Attardo GM, Hansen IA, Raikhel AS,
Journal:J Biol Chem
PubMed ID:16490782
'Ingestion of blood is required for vector mosquitoes to initiate reproductive cycles determining their role as vectors of devastating human diseases. Nutritional signaling plays a pivotal role in regulating mosquito reproduction. Transcription of yolk protein precursor genes is repressed until mosquitoes take blood. Previously, we have shown that to signal ... More
Inwardly rectifying K+ (Kir) channels in Drosophila. A crucial role of cellular milieu factors Kir channel function.
Authors: Döring Frank; Wischmeyer Erhard; Kühnlein Ronald P; Jäckle Herbert; Karschin Andreas;
Journal:J Biol Chem
PubMed ID:11964404
'Three cDNAs encoding inwardly rectifying potassium (Kir) channels were isolated from Drosophila melanogaster. The protein sequences of Drosophila KirI (dKirI) and dKirII are moderately (<44%) and dKirIII sequence is weakly (<27%) identical to human Kir channel subunits. During fly development, five dKir channel transcripts derived from three genes are differentially ... More
Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms.
Authors:Fujita N, Takebayashi S, Okumura K, Kudo S, Chiba T, Saya H, Nakao M
Journal:Mol Cell Biol
PubMed ID:10454587
DNA methylation of promoter-associated CpG islands is involved in the transcriptional repression of vertebrate genes. To investigate the mechanisms underlying gene inactivation by DNA methylation, we characterized a human MBD1 protein, one of the components of MeCP1, which possesses a methyl-CpG binding domain (MBD) and cysteine-rich (CXXC) domains. Four novel ... More
A baculovirus superinfection system: efficient vehicle for gene transfer into drosophila S2 cells.
Authors:Lee DF, Chen CC, Hsu TA, Juang JL
Journal:J Virol
PubMed ID:11090187
The baculovirus expression vector system is considered to be a safe, powerful, but cell-lytic heterologous protein expression system in insect cells. We show here that there is a new baculovirus system for efficient gene transfer and expression using the popular and genetically well-understood Drosophila S2 cells. The recombinant baculovirus was ... More
The eukaryotic DNMT2 genes encode a new class of cytosine-5 DNA methyltransferases.
Authors:Tang LY, Reddy MN, Rasheva V, Lee TL, Lin MJ, Hung MS, Shen CK,
Journal:J Biol Chem
PubMed ID:12819212
DNMT2 is a subgroup of the eukaryotic cytosine-5 DNA methyltransferase gene family. Unlike the other family members, proteins encoded by DNMT2 genes were not known before to possess DNA methyltransferase activities. Most recently, we have shown that the genome of Drosophila S2 cells stably expressing an exogenous Drosophila dDNMT2 cDNA ... More
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