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引用和文献 (20)
Invitrogen™
pSecTag2 A、B 和 C 哺乳动物表达载体
pSecTag2 和 pSecTag2/Hygro 是哺乳动物表达载体，设计用于融合蛋白的分泌、纯化和检测。每个载体在三个读码框内具有大量多克隆位点，以简化带有 N 端分泌信号的框内克隆。这些载体（图了解更多信息
| 货号 | 数量 |
|---|---|
| V90020 | 20 μg |
货号 V90020
价格（CNY)
16,028.00
20 µg
数量:
20 μg
价格（CNY)
16,028.00
20 µg
pSecTag2 和 pSecTag2/Hygro 是哺乳动物表达载体，设计用于融合蛋白的分泌、纯化和检测。每个载体在三个读码框内具有大量多克隆位点，以简化带有 N 端分泌信号的框内克隆。这些载体（图 1）具有以下特性：
•小鼠 Ig κ 链 V-J2-C 区域的分泌信号可实现重组蛋白的高效分泌（图 2）
• 巨细胞病毒 (CMV) 启动子能实现高水平构成性表达
• C 端多组氨酸 (6xHis) 标记可采用镍螯合树脂进行快速纯化，以及采用抗 His（C 端）抗体进行检测
• C 端 c-myc 表位可采用抗 myc 抗体进行检测
• 牛生长激素 (BGH) 聚腺苷酸化信号和转录终止序列可增强 mRNA 的稳定性
• SV40 复制区序列用于在表达大 T 抗原的细胞系中进行附加型复制和简单的载体救援（例如，COS-1，COS-7）
pSecTag2 载体携带 Zeocin™ 抗性基因，可对哺乳动物细胞进行高性价比筛选。Zeocin™ 筛选试剂也可用于大肠杆菌。
pSecTag2/Hygro 载体具有潮霉素 B 抗性基因，用于稳定的哺乳动物细胞系筛选。
•小鼠 Ig κ 链 V-J2-C 区域的分泌信号可实现重组蛋白的高效分泌（图 2）
• 巨细胞病毒 (CMV) 启动子能实现高水平构成性表达
• C 端多组氨酸 (6xHis) 标记可采用镍螯合树脂进行快速纯化，以及采用抗 His（C 端）抗体进行检测
• C 端 c-myc 表位可采用抗 myc 抗体进行检测
• 牛生长激素 (BGH) 聚腺苷酸化信号和转录终止序列可增强 mRNA 的稳定性
• SV40 复制区序列用于在表达大 T 抗原的细胞系中进行附加型复制和简单的载体救援（例如，COS-1，COS-7）
pSecTag2 载体携带 Zeocin™ 抗性基因，可对哺乳动物细胞进行高性价比筛选。Zeocin™ 筛选试剂也可用于大肠杆菌。
pSecTag2/Hygro 载体具有潮霉素 B 抗性基因，用于稳定的哺乳动物细胞系筛选。
仅供科研使用。不可用于诊断程序。
规格
输送类型转染
适用于（应用）分泌型表达
产品类型哺乳动物表达载体
数量20 μg
选择试剂（真核生物）Zeocin™
载体pSec
克隆方法限制性内切酶/MCS
促进剂CMV
蛋白标记His 标签 (6x)、c-Myc 抗原决定簇标签、IgK 前导序列, c-Myc Epitope Tag, IgK Leader Sequence
Unit Size20 µg
内容与储存
20 μg 每种载体和 PSA 表达对照品均以超螺旋化冻干形式提供。储存于 -20°C。如果储存恰当，载体可以确保 6 个月稳定。
常见问题解答 (FAQ)
我使用了pSecTag2载体，仅观察到目的基因在胞浆中表达，未见分泌型表达。你们能提供一些帮助么？
我能够在大肠杆菌中扩增包含Tn5基因的pSecTag2载体么？
我发现pSectag2载体携带了EM7启动子驱动的Zeocin抗生素抗性基因。将这些载体置于大肠杆菌中扩增时，我能使用Zeocin抗性来进行筛选么？
pSecTag2和pSecTag2/Hygro载体之间的区别是什么？
我希望表达一个分泌蛋白。你们对此有何建议？
引用和文献 (20)
引用和文献
Abstract
Neuropilin-1 binds vascular endothelial growth factor 165, placenta growth factor-2, and heparin via its b1b2 domain.
Journal:J Biol Chem
PubMed ID:11986311
'Neuroplin-1 (NRP1), a receptor for vascular endothelial growth factor (VEGF) family members, has three distinct extracellular domains, a1a2, b1b2, and c. To determine the VEGF(165) and placenta growth factor 2 (PlGF-2)-binding sites of NRP1, recombinant NRP1 domains were expressed in mammalian cells as Myc-tagged, soluble proteins, and used in co-precipitation
Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10.
Journal:J Biol Chem
PubMed ID:14701821
'The adhesive interactions of cells with laminins are mediated by integrins and non-integrin-type receptors such as alpha-dystroglycan and syndecans. Laminins bind to these receptors at the C-terminal globular domain of their alpha chains, but the regions recognized by these receptors have not been mapped precisely. In this study, we sought
Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5).
Journal:J Biol Chem
PubMed ID:10956661
'Based on sequence homology with a previously cloned human GlcNAc 6-O-sulfotransferase, we have identified an open reading frame (ORF) encoding a novel member of the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family termed GST-5 on the human X chromosome (band Xp11). GST-5 has recently been characterized as a novel GalNAc 6-O-sulfotransferase termed chondroitin
Plexin-B1 directly interacts with PDZ-RhoGEF/LARG to regulate RhoA and growth cone morphology.
Journal:Neuron
PubMed ID:12123608
'Plexins are widely expressed transmembrane proteins that, in the nervous system, mediate repulsive signals of semaphorins. However, the molecular nature of plexin-mediated signal transduction remains poorly understood. Here, we demonstrate that plexin-B family members associate through their C termini with the Rho guanine nucleotide exchange factors PDZ-RhoGEF and LARG. Activation
Death receptor 5, a new member of the TNFR family, and DR4 induce FADD- dependent apoptosis and activate the NF-kappaB pathway.
Journal:Immunity
PubMed ID:9430227
'Death receptor 4 (DR4) is a recently described receptor for the cytotoxic ligand TRAIL that reportedly uses a FADD-independent pathway to induce apoptosis and does not activate the NF-kappaB pathway. We have isolated a new member of the tumor necrosis factor receptor (TNFR) family, designated DR5, which bears a high