Zip Alexa Fluor™ 快速抗体标记试剂盒
Zip Alexa Fluor™ 快速抗体标记试剂盒
Invitrogen™

Zip Alexa Fluor™ 快速抗体标记试剂盒

使用 Zip Alexa Fluor 快速抗体标记试剂盒仅需 15 分钟即可生成用于荧光显微镜检测、流式细胞分析、IHC、ICC 等分析的抗体偶联物。
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货号标签或染料激发/发射
Z11235Alexa Fluor 647650/665
Z11233Alexa Fluor 488490/525
Z11234Alexa Fluor 555555/580
货号 Z11235
价格（CNY)
5,269.00
3 labelings
添加至购物车
标签或染料:
Alexa Fluor 647
激发/发射:
650/665
价格（CNY)
5,269.00
3 labelings
添加至购物车

Invitrogen Zip Alexa Fluor 快速抗体标记试剂盒可利用 Alexa Flour 染料有效快速标记您的珍贵抗体，以在 15 分钟内获取可直接使用的抗体偶联物，回收率为 100%。每个试剂盒含有执行三次 100 μg 抗体单独标记反应所需的全部组分。只需向含有 Zip 缓冲液的组分 A 中加入 1 mL 水，加入 100 μg 抗体，孵育 15 分钟，即可使用您的抗体。这些偶联物适用于多种应用，包括流式细胞分析、荧光显微镜检测、免疫组织化学技术 (IHC)、初步检测、ELISA、免疫细胞化学技术 (ICC) 和间接 FISH。

使用我们的 Invitrogen Zip Alexa Fluor 快速抗体标记试剂盒只需 15 分钟即可生成即用型抗体偶联物。这些试剂盒可帮助您有效地使用各种 Alexa Flour 染料标记珍贵的抗体。

Zip Alexa Fluor 快速抗体标记试剂盒的重要特点：
· 快速 — 抗体偶联物在 15 min 内即可使用
· 高效 — 100% 抗体回收率
· 多功能性 — 试剂盒适用于多种应用
· 易于使用 — 无需化学或偶联经验
· 灵活性 — 可从 Alexa 488、555 和 647 中选择

每个试剂盒包含执行 3 次独立的 100 µg 抗体标记反应所需的一切组分。所得偶联物是多种应用的理想之选，包括流式细胞分析、荧光显微镜检测、免疫组织化学技术 (IHC)、初步检测、ELISA、免疫细胞化学技术 (ICC) 和间接 FISH。

使用 Zip Alexa Fluor 快速抗体标记试剂盒的优势

特异性和多功能性
直接用荧光基团标记一抗，通常产生的背景荧光更少，非特异性结合比例更低。只要直接标记了兼容的荧光基团，同种型或来自相同物种的多种一抗可用在同一实验中。

耐用性
与传统染料相比，Alexa Fluor 染料更明亮、光稳定性更好，且在 pH 值 4 至 10 之间的 pH 值耐受性更强。在使用 Alexa Fluor 染料时，无需分子内淬灭即可实现更高水平的标记。

可以实现定制
库存列表中找不到您所需的内容？我们可以为您制备定制抗体偶联物。我们的定制偶联服务高效且保密, 而且我们绝对保证质量。我们经过ISO 9001:2000认证。

仅供科研使用。不可用于诊断程序。
规格
颜色远红外
激发/发射650/665
套装内容含有反应性荧光标记，3 样品瓶；碳酸氢钠，3 样品瓶
标签类型Alexa Fluor 染料
标记规模100 μg
产品线Alexa Fluor™
产品类型Labeling Kit
数量3 次反应
化学反应性胺
标记目标IgG
标签或染料Alexa Fluor 647
Unit Size3 labelings
内容与储存
干燥避光冷冻（-5°C 至 +30°C）储存。

常见问题解答 (FAQ)

With the Zip Alexa Fluor Rapid Antibody Labeling kits, can I store the labeled antibody after the 15 mins reaction?

No, it is intended for immediate use. If you wish to store your labeled antibody after the reaction, it should undergo a post-reaction clean-up using either size exclusion resin (such as Bio-Rad BioGel P-30) or Dye Removal Columns (Cat No. 22858).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do the Zip Alexa Fluor Rapid Antibody Labeling kits not require a post-reaction purification step?

This is because we have optimized the amount of dye to label 100 µg of antibody per reaction and after the incubation time of 15 mins, most of the reactive dye is either bound or unreactive due to spontaneous hydrolysis, leaving little or no reactive dye to label your sample.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am planning to use one of the Zip Alexa Fluor Rapid Antibody Labeling kits. My antibody is shipped in Tris buffer with BSA added as a stabilizer. Do I need to remove the BSA or Tris prior to the reaction?

Yes, the BSA and any component with a primary amine (such as the Tris buffer) would react with the amine-reactive Alexa Fluor dyes used in these labeling kits, resulting in less optimal labeling of the antibody. For small volume purification of the antibody, you may use either Nab Protein A/G Spin Kit, 0.2 mL (Cat No. 89950), Protein A/G Magnetic Agarose Beads (Cat No. 78609), or Melon Gel IgG Spin Purification Kit (Cat No. 45206).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For the Zip Alexa Fluor Rapid Antibody Labeling kits, why does the antibody have to be at a 1 mg/mL concentration?

The 1 mg/mL concentration of the antibody (100 µg of antibody in a 100 µL volume) allows for optimal reaction kinetics. A more dilute solution of antibody would exhibit suboptimal labeling due to a greater amount of reactive dye lost to spontaneous hydrolysis; the reactive group on the dyes is labile in aqueous solutions.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is included in the Zip Alexa Fluor Rapid Antibody Labeling kits?

Each kit contains 3 vials of solid sodium bicarbonate (Zip buffer reagent, Component A), 3 vials of the Alexa Fluor reactive dye (Component B), and a Quick Reference Card. Sufficient reagents are provided to label 100 µg of antibody per reaction (100 µL of a 1 mg/mL antibody solution), 3 reactions per kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.