AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer
AmpliTaq&trade; 360 Buffer, 25 mM MgCl<sub>2</sub> and 360 GC Enhancer
Applied Biosystems™

AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer

Green features
The Applied Biosystems AmpliTaq 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs.
Have Questions?
產品號碼Quantity
43988481.5 mL
產品號碼 4398848
價格 (HKD)
822.00
Each
新增至購物車
Quantity:
1.5 mL
Request bulk or custom format
價格 (HKD)
822.00
Each
新增至購物車
The Applied Biosystems AmpliTaq 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA Polymerase for situations when the reagents supplied with the enzyme are not sufficient for the experimental needs. When used with or without the optional 360 GC Enhancer, this buffer amplifies a vast range of DNA sequence contexts. AmpliTaq 360 DNA Polymerase delivers 360° coverage for a full range of targets.

The AmpliTaq 360 Buffer Kit is designed for use with challenging targets including AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can be reproducibly amplified with a single reagent under standardized conditions. Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 Polymerase as a superior-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets.

Features

  • Optimized for the broad range of targets
  • Robust amplification of GC-rich sequences with 360 GC Enhancer
  • Achieves high quality sequencing data

Notes

  • GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.
  • See user's manual or package insert for limited label license and trademark information.
For Research Use Only. Not for use in diagnostic procedures.
規格
Green FeaturesSustainable packaging
PolymeraseAmpliTaq 360 DNA Polymerase
Product TypeGC Enhancer
Quantity1.5 mL
Reaction FormatKit
Shipping ConditionRoom Temp or Wet Ice
Volume1.5 mL
Concentration5 U/μL
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceHigh
Reaction SpeedStandard
Unit SizeEach
內容物與存放
• 10X AmpliTaq 360 Buffer, 1.5 mL
• 25 mM MgCl2, 1.5 mL
• 360 GC Enhancer, 1.5 mL

Store at -15°C to -25°C.
Product is guaranteed up to 12 months as indicated by the expiration date.

常見問答集 (常見問題)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer FAQs