GlutaMAX™ Supplement

Gibco GlutaMAX Supplement is an L-alanyl-L-glutamine dipeptide alternative to L-glutamine that exhibits increased stability, leading to improved cell health. L-Glutamine is an amino acid required in cell culture for the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. GlutaMAX Supplement is suitable for adherent and suspension culture of mammalian cells, with no adaptation required. It is formulated as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl.

Compared to L-glutamine, GlutaMAX Supplement:

• Minimizes toxic ammonia build-up
• Improves cell viability and growth
• Remains stable across a wide range of temperatures

Minimizes toxic ammonia build-up

Unlike L-glutamine, GlutaMAX Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.

Improves cell viability and growth

Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX Supplement that is not possible with L-glutamine.

Remains stable across a wide range of temperatures

GlutaMAX Supplement is room-temperature stable and ready to use. It retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX Supplement is even stable at 37°C for a week or more.