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Invitrogen
DYKDDDDK Tag Monoclonal Antibody (FG4R), eBioscience™
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FIGURE: 1 / 4
DYKDDDDK Tag Antibody (14-6681-80) in ICC/IF
Immunofluorescent analysis of DYKDDDDK Tag was performed using 70% confluent HA-H3-DYKDDDDK transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DYKDDDDK Tag Monoclonal Antibody (FG4R), eBioscience™ (Product # 14-6681-80) at 5 µg/mL and... View More
Product Details
14-6681-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
C. elegans
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
FG4R
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This FG4R monoclonal antibody reacts with the DYKDDDDDK tag. This motif is frequently fused to the N- or C-terminus of proteins to facilitate purification (e.g., affinity chromatography and immunoprecipitation) and/or detection. This antibody recognizes the DYKDDDDK anywhere within the protein of interest (i.e., N-terminus, C-terminus, or internal).
Applications Tested: This FG4R antibody has been tested by immunoblotting of reduced cell lysate expressing a DYKDDDDK-tagged protein at less than or equal to 1 µg/ mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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