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Invitrogen
SPAM1 Recombinant Rabbit Monoclonal Antibody (4N0R3)
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SPAM1 Antibody (MA5-51314) in IHC (P)
Immunohistochemistry analysis of SPAM1 in paraffin-embedded human testis. Samples were incubated with SPAM1 Recombinant monoclonal antibody (Product # MA5-51314) using a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-51314
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4N0R3
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
0.55 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hyal-PH20; Hyaluronidase PH-20; Hyaluronoglucosaminidase PH-20; MGC26532; Sperm adhesion molecule 1; Sperm surface antigen 2B1; Sperm surface protein PH-20
Gene Aliases: Ph20; Spam; Spam1
UniProt ID: (Rat) Q62803
Entrez Gene ID: (Rat) 117037
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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