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Invitrogen
PAG1 Polyclonal Antibody
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FIGURE: 1 / 1
PAG1 Antibody (PA1-19375) in WB
Western blotting analysis of human PAG using rabbit polyclonal antibody PAb (409) (Product # PA1-19375) on lysates of Daudi and Ramos cell line under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of rabbit anti-PAG monoclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. PAG was detected around 65kDa.
Product Details
PA1-19375
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes intracellular part of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Target Information
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Csk-binding protein; phosphoprotein associated with glycosphingolipid microdomains 1; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; phosphoprotein transmembrane adaptor 1; phosphoprotein-associated with GEMs; Transmembrane adapter protein PAG; transmembrane adaptor protein PAG; Transmembrane phosphoprotein Cbp
Gene Aliases: CBP; F730007C19Rik; PAG; PAG1
UniProt ID: (Human) Q9NWQ8, (Mouse) Q3U1F9
Entrez Gene ID: (Human) 55824, (Mouse) 94212
adaptive immune response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
epidermal growth factor receptor signaling pathway
positive regulation of signal transduction
intracellular signal transduction
T cell receptor signaling pathway
regulation of T cell activation
negative regulation of T cell activation
immune system process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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