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Invitrogen
Actin Polyclonal Antibody
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FIGURE: 1 / 6
Actin Antibody (PA5-11570) in ICC/IF
Immunofluorescence analysis of Actin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Actin Polyclonal Antibody (Product # PA5-11570) at 1:50 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-11570
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human Actin (ACTB/ACTC)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.8 mg/mL
Amount
720 µg
Purification
Size-exclusion - Dialysis, Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, chicken, canine, equine, hamster, porcine, non-human primate, rat, rabbit and Xenopus, yeast and zebrafish based on sequence homology.
Target Information
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ACT2; actin 2; actin 7; actin alpha cardiac; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 1; Actin, cytoplasmic 2; Actin, gamma-enteric smooth muscle; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alphac-actin; AtACT7; Atcb; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; DEFORMED ROOT HAIRS 1; DER1; ENHANCER OF LRX1 2; ENL2; FIZ2; FRIZZY AND KINKED SHOOTS 2; Gamma-2-actin; Gamma-actin; LIGHT STRESS-REGULATED 2; LSR2; MYH9.2; MYH9_2; pan actin; RP5-1068B5.2; Smooth muscle gamma-actin
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTG; ACTG1; ACTG2; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; beta-actin; E430023M04Rik; E51; GIG46; SMalphaA; SMGA
UniProt ID: (Mouse) P68134, (Mouse) P62737, (Mouse) P60710, (Mouse) P68033, (Mouse) P63260, (Mouse) P63268
Entrez Gene ID: (Mouse) 11459, (Mouse) 11475, (Mouse) 11461, (Mouse) 11464, (Mouse) 11465, (Mouse) 11468
skeletal muscle thin filament assembly
skeletal muscle fiber development
muscle contraction
regulation of blood pressure
vascular smooth muscle contraction
metabolic process
actin filament-based movement
actomyosin structure organization
actin-myosin filament sliding
negative regulation of apoptotic process
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
actin-mediated cell contraction
sarcomere organization
platelet aggregation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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