Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Phospho-GRB10 (Ser476) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 5
Phospho-GRB10 (Ser476) Antibody (PA5-104597) in IHC (P)
Immunohistochemistry analysis of Phospho-GRB10 (Ser476) in mouse kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-GRB10 (Ser476) polyclonal antibody (Product # PA5-104597) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-104597
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GRB10(Accession Q13322), corresponding to amino acid residues around phosphorylated Ser476.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of GRB10 only when phosphorylated at Ser476.
Target Information
GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP, and a proline-rich N terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. GRB10 maps to mouse chromosome 11, in close proximity to the EGF receptor. Similarly, GRB7 maps to the same mouse chromosome near the EGF receptor-related protein HER2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: GRB10 adapter protein; GRB10 adaptor protein; Growth factor receptor-bound protein 10; Insulin receptor-binding protein Grb-IR; maternally expressed gene 1; Maternally expressed gene 1 protein; OTTHUMP00000197988; OTTHUMP00000197989; OTTHUMP00000197990; OTTHUMP00000197991; OTTHUMP00000197993; OTTHUMP00000197994
Gene Aliases: 5730571D09Rik; AI325020; Grb-10; GRB-IR; GRB10; GRBIR; IRBP; KIAA0207; MEG1; mKIAA0207; RGD1566234; RSS
UniProt ID: (Human) Q13322, (Mouse) Q60760
Entrez Gene ID: (Human) 2887, (Mouse) 14783, (Rat) 498416
negative regulation of Wnt signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
negative regulation of phosphorylation
negative regulation of glucose import
negative regulation of insulin receptor signaling pathway
insulin-like growth factor receptor signaling pathway
signal transduction
insulin receptor signaling pathway
positive regulation of signal transduction
response to insulin
positive regulation of phosphorylation
negative regulation of glycogen biosynthetic process
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support