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Invitrogen
Methyl-YAP1 (Lys342) Polyclonal Antibody
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FIGURE: 1 / 1
Methyl-YAP1 (Lys342) Antibody (PA5-116873) in WB
Western blot analysis of Methyl-YAP1 (Lys342) in extracts of various cell lines. Samples were incubated with Methyl-YAP1 (Lys342) Polyclonal antibody (Product # PA5-116873) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-116873
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic monomethylated peptide around K342 of human YAP1 (NP_0011236171)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.35 mg/mL
Amount
35 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse kidney, Rat liver
Immunogen sequence: SPKCQ
Target Information
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; Yes-associated protein 1; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog
Gene Aliases: AI325207; Yap; YAP1; YAP65; Yki; Yorkie
UniProt ID: (Rat) Q2EJA0, (Mouse) P46938
Entrez Gene ID: (Rat) 363014, (Mouse) 22601
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
chromatin binding
transcription coactivator activity
transcription corepressor activity
protein C-terminus binding
transcription regulatory region DNA binding
proline-rich region binding
protein binding
transcription cofactor
cell morphogenesis
vasculogenesis
embryonic heart tube morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
DNA damage induced protein phosphorylation
cell proliferation
positive regulation of cell proliferation
regulation of keratinocyte proliferation
keratinocyte differentiation
negative regulation of epithelial cell differentiation
notochord development
somatic stem cell population maintenance
hippo signaling
regulation of cell proliferation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of organ growth
paraxial mesoderm development
lateral mesoderm development
contact inhibition
bud elongation involved in lung branching
lung epithelial cell differentiation
regulation of canonical Wnt signaling pathway
cellular response to retinoic acid
cellular response to gamma radiation
regulation of stem cell proliferation
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of canonical Wnt signaling pathway
positive regulation of stem cell population maintenance
negative regulation of nucleic acid-templated transcription
negative regulation of stem cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
regulation of neurogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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