For reproducible results, choose reliable qPCR reagents

Optimized real-time PCR (qPCR) analysis enables sensitive and specific quantification of nucleic acids. Reagent selection plays a critical role in any qPCR or RT-qPCR protocol to help ensure optimal performance and reliable results. Set your analysis up for success by choosing an Applied Biosystems real-time PCR master mix or reagent.

 

Real-time PCR master mixes, sometimes referred to as "mastermixes", "super mixes", or "ready mixes", are concentrated, pre-mixed solutions that contain many components required for a qPCR reaction, including buffer, enzymes, and dNTPs. With over 25 years of experience innovating real-time PCR reagents, Thermo Fisher Scientific has developed optimized master mixes for almost any qPCR workflow. We offer master mix formulations tailored to meet the unique requirements of different applications, such as gene expression,  genotyping, and pathogen detection.


Real-Time PCR reagent & master mix options

TaqMan qPCR and RT-qPCR master mixes

Explore TaqMan master mixes for probe-based qPCR detection in gene expression, genotyping, microbial detection, and multiplex research applications. Compare options and find the right solution for your workflow

TaqPath qPCR and RT-qPCR Master Mixes

Explore TaqPath master mixes engineered for assay development, high-performance multiplexing, and robust qPCR and RT-qPCR workflows. Compare options and find the right solution for post-discovery and translational applications

SYBR Green qPCR master mixes

Explore SYBR Green qPCR master mixes engineered for sensitivity, specificity, and reproducibility. Compare options and find the right SYBR mix for your workflow and application needs.

qPCR reagents for gene expression analysis

For accurate detection and quantification of gene expression targets, view our recommended TaqMan and SYBR Green master mixes for gene expression analysis.

Other reagents & kits

Explore more products including HRM master mixes, exogenous controls, sample-to-results kits, instrument calibration and verification kits, and Lyo-Ready master mixes that support lyophilized assay developers.

1-step RT-qPCR master mixes

Explore 1-step RT-qPCR master mixes designed to simplify RNA detection workflows across applications including gene expression, microbial detection,  and multiplexing.

For Research Use Only. Not for use in diagnostic procedures.