Invitrogen

CD140a (PDGFRA) Monoclonal Antibody (APA5), PerCP-eFluor™ 710, eBioscience™

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产品信息

46-1401-82

应用
建议稀释比
已发表文章

免疫组化 (IHC)

-
查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

-
查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.125 µg/test
查看25篇已发表文章 25篇已发表文章
产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

推荐的同型对照

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

APA5

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱 spectra

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2815148

产品详细信息

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet-derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of an antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

靶标信息

PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; PDGF-R-alpha; pdgfr-a; PDGFR-alpha; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor alpha

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基因别名: AI115593; CD140a; Pdgfr-2; Pdgfra

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UniProt ID: (Mouse) P26618

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Entrez Gene ID: (Mouse) 18595

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功能
nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity platelet-derived growth factor alpha-receptor activity vascular endothelial growth factor-activated receptor activity platelet-derived growth factor receptor binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding vascular endothelial growth factor binding protein homodimerization activity phosphatidylinositol 3-kinase binding platelet-derived growth factor binding transmembrane signal receptor
参与通路
luteinization in utero embryonic development hematopoietic progenitor cell differentiation protein phosphorylation chemotaxis transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cytosolic calcium ion concentration multicellular organism development estrogen metabolic process positive regulation of cell proliferation female gonad development anatomical structure morphogenesis organ morphogenesis negative regulation of platelet activation positive regulation of phospholipase C activity phosphorylation cell migration peptidyl-tyrosine phosphorylation signal transduction involved in regulation of gene expression extracellular matrix organization lung development adrenal gland development positive regulation of cell migration male genitalia development Leydig cell differentiation cellular response to reactive oxygen species platelet-derived growth factor receptor-alpha signaling pathway cellular response to platelet-derived growth factor stimulus positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway wound healing odontogenesis of dentin-containing tooth positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication protein autophosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation embryonic digestive tract morphogenesis embryonic cranial skeleton morphogenesis skeletal system morphogenesis regulation of chemotaxis cardiac myofibril assembly palate development face morphogenesis cell chemotaxis retina vasculature development in camera-type eye positive regulation of ERK1 and ERK2 cascade platelet aggregation cellular response to amino acid stimulus metanephric glomerular capillary formation regulation of mesenchymal stem cell differentiation
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