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Invitrogen
CD140a (PDGFRA) Monoclonal Antibody (APA5), eBioscience™
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FIGURE: 1 / 94
CD140a (PDGFRA) Antibody (14-1401-82) in Flow
Knockout of CD140a (PDGFRA) was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR465552_SGM) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD140a (PDGFRA) was performed by staining NIH 3T3 CD140a (PDGFRA) Knock out cells with 1 µg Rat IgG2a kappa Isotype Control (eBR2a), eBioscience™ (Product # 14-4321-85, yellow histogram) or 1 µg CD140a (PDGFRA) Monoclonal Antibody (APA5), eBioscience™ (Product # 14-1401-82, blue histogram) followed by Goat anti-Ra... View More
Product Details
14-1401-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
APA5
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.
Applications Reported: The APA5 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified APA5 in functional assays).
Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of the NIH/3T3 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; PDGF-R-alpha; pdgfr-a; PDGFR-alpha; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor alpha
Gene Aliases: AI115593; CD140a; Pdgfr-2; Pdgfra
UniProt ID: (Mouse) P26618
Entrez Gene ID: (Mouse) 18595
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
platelet-derived growth factor alpha-receptor activity
vascular endothelial growth factor-activated receptor activity
platelet-derived growth factor receptor binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein complex binding
vascular endothelial growth factor binding
protein homodimerization activity
phosphatidylinositol 3-kinase binding
platelet-derived growth factor binding
transmembrane signal receptor
luteinization
in utero embryonic development
hematopoietic progenitor cell differentiation
protein phosphorylation
chemotaxis
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cytosolic calcium ion concentration
multicellular organism development
estrogen metabolic process
positive regulation of cell proliferation
female gonad development
anatomical structure morphogenesis
organ morphogenesis
negative regulation of platelet activation
positive regulation of phospholipase C activity
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
signal transduction involved in regulation of gene expression
extracellular matrix organization
lung development
adrenal gland development
positive regulation of cell migration
male genitalia development
Leydig cell differentiation
cellular response to reactive oxygen species
platelet-derived growth factor receptor-alpha signaling pathway
cellular response to platelet-derived growth factor stimulus
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
wound healing
odontogenesis of dentin-containing tooth
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of DNA replication
protein autophosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of fibroblast proliferation
embryonic digestive tract morphogenesis
embryonic cranial skeleton morphogenesis
skeletal system morphogenesis
regulation of chemotaxis
cardiac myofibril assembly
palate development
face morphogenesis
cell chemotaxis
retina vasculature development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
cellular response to amino acid stimulus
metanephric glomerular capillary formation
regulation of mesenchymal stem cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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