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Invitrogen
Ly-6G Monoclonal Antibody (1A8-Ly6g), Alexa Fluor™ 700, eBioscience™
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图: 1 / 26
Ly-6G Antibody (56-9668-82) in Flow
C57BL/6 mouse bone marrow cells were stained with Ly-6C Monoclonal Antibody, PE (Product # 12-5932-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 700 (Product # 56-4321-80) (left) or 0.25 µg of Ly-6G Monoclonal Antibody, Alexa Fluor 700 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
产品信息
56-9668-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
1A8-Ly6g
偶联物
激发/发射光谱
696/719 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown.
Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity.
Applications Reported: This 1A8-Ly6G antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 1A8-Ly6G antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
靶标信息
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow. Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Ly-6G; Ly-6G.1; Lymphocyte antigen 6G
基因别名: Gr-1; Gr1; Ly-6G; Ly6g
UniProt ID: (Mouse) P35461
Entrez Gene ID: (Mouse) 546644
