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Invitrogen
APS Polyclonal Antibody
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APS Antibody (PA1-1062) in ICC/IF
Immunofluorescence analysis of APS was performed using 70% confluent log phase Neuro-2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with APS Rabbit Polyclonal Antibody (Product # PA1-1062) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More
产品信息
PA1-1062
产品规格
种属反应
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues E(605) A T L G R A R A V E N Q Y S(619) from mouse APS.
偶联物
形式
Liquid
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-1062 detects APS from mouse samples.
PA1-1062 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~ 84 kDa protein corresponding to APS from recombinant mouse protein and 3T3L1 extracts.
The PA1-1062 immunogen is a synthetic peptide corresponding to residues E(605)A T L G R A R A V E N Q Y S(619) from mouse APS. This peptide (Cat. # PEP-246) can be used in neutralization and control experiments.
靶标信息
APS (adapter protein with Pleckstrin homology and Src homology 2 domains) a member of the Lnk family, is an adaptor protein that is involved in B-cell signaling, insulin signaling, cytoskeletal reorganization. It is tyrosine phosphorylated by JAK2, KIT and other kinases during B-cell receptor stimulation of many different cytokines, chemokines and leukokines. APS has been shown to inhibit the JAK-STAT pathway in collaboration with c-Cbl. It is located in the cytoplasm in pre-PDGF (platelet derived growth factor) stimulated cells and localizes to the plasma membrane and peripheral regions upon PDGF stimulation. This protein is expressed in B-cells and mast cells when detected in haematopoietic cell lines yet is also expressed in many different tissues including spleen, prostrate, testis, uterus, small intestine and skeletal muscle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Adapter protein with pleckstrin homology and Src homology 2 domains; Alpha-1-antitrypsin; SH2 and PH domain-containing adapter protein APS; SH2B adapter protein 2
基因别名: Aps; Sh2b2
UniProt ID: (Mouse) Q9JID9
Entrez Gene ID: (Mouse) 23921
