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Rockland
GGA1 Polyclonal Antibody
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GGA1 Antibody (600-401-853) in WB
Western blot using Rocklands Affinity Purified anti-GGA1 antibody shows detection of bands at ~100 kDa corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells over- expressing the recombinant protein (arrowhead). Approximately 35 µg of lysate was separated on a 4-20% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4° followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt anti-Rb ... View More
产品信息
600-401-853
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-360 of Human GGA-1.
偶联物
形式
Liquid
浓度
1.35 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human GGA1 protein. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Cross reactivity may occur with GGA1 from bovine (87% homology), mouse (82% homology) and rat (76% homology) sources. The immunizing sequence is present only in isoform 1 of this protein, therefore cross reactivity with splice variants is not expected. Cross reactivity with GGA1 homologues from other sources has not been determined.
靶标信息
This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ADP-ribosylation factor binding protein 1; ADP-ribosylation factor-binding protein GGA1; gamma-adaptin related protein 1; Gamma-adaptin-related protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 1; Golgi-localized, gamma ear-containing, ARF-binding protein 1
基因别名: GGA1
UniProt ID: (Human) Q9UJY5
Entrez Gene ID: (Human) 26088
