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Invitrogen
SIGLEC8 Polyclonal Antibody
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SIGLEC8 Antibody (PA5-87961) in WB
Western blot analysis of SIGLEC8 in extracts of various cell lines. Samples were incubated with SIGLEC8 Polyclonal antibody (Product # PA5-87961) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-87961
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human Siglec-8 (NP_0552572)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.05 mg/mL
规格
105 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MEGDRQYGDG YLLQVQELVT VQEGLCVHVP CSFSYPQDGW TDSDPVHGYW FRAGDRPYQD APVATNNPDR EVQAETQGRF QLLGDIWSND CSLSIRDARK RDKGSYFFRL ERGSMKWSYK SQLNYKTKQL SVFVTALTHR PDILILGTLE SGHSRNLTCS VPWACKQGTP PMISWIGASV SSPGPTTARS SVLTLTPKPQ DHGTSLTCQV TLPGTGVTTT STVRLDVSYP PWNLTMTVFQ GDATASTALG NGSSLSVLEG QSLRLVCAVN SNPPARLSWT RGSLTLCPSR SSNPGLLELP RVHVRDEGEF TCRAQNAQGS QHISLSLSLQ NEGTGTSRPV SQVTLAA; Positive Samples: HL-60, 293T, Mouse liver, Rat blood; Cellular Location: Membrane, Single-pass type I membrane protein
靶标信息
SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC59785; Siglec
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