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Invitrogen
HEF1 Polyclonal Antibody
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FIGURE: 1 / 6
HEF1 Antibody (PA5-79727) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of HEF1 in Hela cells using HEF1 Polyclonal Antibody (Product # PA5-79727). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualized using a fluorescence microscope and filter sets appropriate for the label used.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79727
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human HEF1 recombinant protein (Position: K273-E421).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human Daudi whole cell, monkey COS-7 whole cell, human MCF-7 whole cell, rat spleen tissue, mouse spleen tissue. IHC: human breast cancer tissue. ICC/IF: Hela cell. Flow: A549 cell.
Target Information
HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cas scaffolding protein family member 2; cas-like docking; Crk-associated substrate lymphocyte-specific protein; Crk-associated substrate related protein Cas-L; CRK-associated substrate-related protein; dJ49G10.2; dJ761I2.1; Enhancer of filamentation 1 p55; neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated gene 9; p130Cas-related protein; pp105; p130Cas-related protein; renal carcinoma antigen NY-REN-12; unnamed protein product
Gene Aliases: CAS-L; CAS2; CASL; CASS2; HEF1; MEF1; Nedd-9; p105
UniProt ID: (Human) Q14511, (Mouse) O35177
Entrez Gene ID: (Human) 4739, (Mouse) 18003, (Rat) 291044
cytoskeleton organization
cell adhesion
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
integrin-mediated signaling pathway
learning or memory
cell migration
positive regulation of cell migration
negative regulation of cell migration
regulation of actin cytoskeleton organization
positive regulation of osteoclast differentiation
actin filament bundle assembly
positive regulation of protein tyrosine kinase activity
cilium disassembly
lymphocyte migration into lymphoid organs
positive regulation of lymphocyte chemotaxis
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of dendritic spine maintenance
positive regulation of cellular protein localization
positive regulation of immunological synapse formation
activation of GTPase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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