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Invitrogen
NPC1 Polyclonal Antibody
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FIGURE: 1 / 1
NPC1 Antibody (PA5-79754) in WB
Western blot analysis of NPC1 in Lane 1: rat pancreas tissue lysate, Lane 2: mouse NIH3T3 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with NPC1 polyclonal antibody (Product # PA5-79754) at a dilution of 0.5 µg/mL (overnight at 4°C), followed b... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79754
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Niemann Pick C1 recombinant protein (Position: A1022-F1278).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat pancreas tissue, mouse NIH3T3 whole cell.
Target Information
Mutations in the Niemann-Pick disease type C1 (NPC1) gene result in a fatal progressive neurodegenerative disorder characterized by an abnormal sequestration of lipids including cholesterol and glycosphingolipids. The NPC1 protein is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolyzed and released as free cholesterol. NPC1, in addition to FTO, MC4R, and PTER has recently been shown to be a new risk loci for early-onset and morbid adult obesity in European populations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ98532; Niemann-Pick C1 protein; Niemann-Pick disease, type C1; Niemann-Pick type C1; solute carrier family 66 member 1; sphingomyelinosis; unnamed protein product
Gene Aliases: A430089E03Rik; Cdig2; D18Ertd139e; D18Ertd723e; lcsd; nmf164; NPC; POGZ; SLC65A1; SLC66A1; spm
UniProt ID: (Human) O15118, (Mouse) O35604
Entrez Gene ID: (Human) 4864, (Mouse) 18145, (Rat) 266732
liver development
endocytosis
autophagy
lysosomal transport
adult walking behavior
bile acid metabolic process
glycoprotein biosynthetic process
response to xenobiotic stimulus
gene expression
cholesterol storage
programmed cell death
sterol transport
sterol metabolic process
negative regulation of macroautophagy
neurogenesis
intestinal cholesterol absorption
cholesterol transport
membrane raft organization
intracellular lipid transport
intracellular cholesterol transport
cholesterol efflux
cholesterol homeostasis
response to cadmium ion
viral entry into host cell
cellular response to steroid hormone stimulus
cellular response to low-density lipoprotein particle stimulus
lysosome to ER cholesterol transport
establishment of protein localization to membrane
negative regulation of epithelial cell apoptotic process
negative regulation of TORC1 signaling
cyclodextrin metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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