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PA1-26846 detects Avidin. PA1-26846 will not react with streptavidin.
PA1-26846 has been successfully used in immunohistochemistry (paraffin) (frozen), ELISA and Western blot procedures.
The PA1-26846 immunogen is Avidin from hen egg white.
Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。