Invitrogen

CD4 Monoclonal Antibody (S3.5), PE-Cyanine7

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CD4 Antibody in Flow Cytometry (Flow) — Human peripheral blood lymphocytes were stained using PE-Cy®7 of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.

产品信息

MHCD0412

应用	建议稀释比	已发表文章
流式细胞分析 (Flow)	Assay-Dependent	查看5篇已发表文章 5篇已发表文章
其他PubMed (Misc)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human
已发表种属	Human
宿主/亚型	Mouse / IgG2a
分类	Monoclonal
类型	Antibody
克隆号	S3.5
抗原	Human CD4
偶联物	PE-Cyanine7 Alexa Fluor 405 Alexa Fluor 488 Alexa Fluor 700 APC APC-Alexa Fluor 750 APC-Cyanine5.5 FITC Pacific Blue Pacific Orange PE PE-Alexa Fluor 610 PE-Alexa Fluor 700 PE-Cyanine5.5 PE-Texas Red PerCP Qdot 605 Qdot 655 Qdot 705 Qdot 800 TRI-COLOR Unconjugated
激发/发射光谱	查看光谱
形式	Liquid
纯化类型	purified
保存液	PBS with 10% sucrose
内含物	0.1% sodium azide
保存条件	4° C, store in dark
RRID	AB_10373809

产品详细信息

The R-phycoerythrin (PE)-Cy7® tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a 488 nm laser without occupying traditional channels associated with FITC and PE.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Analyte Specific Reagent

Flow Cytometry

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Product References

A phase I/II study of a MUC1 peptide pulsed autologous dendritic cell vaccine as adjuvant therapy in patients with resected pancreatic and biliary tumors.

Cancer therapy

Lepisto AJ,Moser AJ,Zeh H,Lee K,Bartlett D,McKolanis JR,Geller BA,Schmotzer A,Potter DP,Whiteside T,Finn OJ,Ramanathan RK

MHCD0412 was used in Flow cytometry/Cell sorting to study the use of a MUC1 peptide pulsed autologous dendritic cell vaccine as adjuvant therapy in patients with resected pancreatic and biliary tumours.

Wed Oct 20 00:00:00 EDT 2021

Increased ratio of Th17 cells to SIGIRR⁺CD4⁺ T cells in peripheral blood of patients with SLE is associated with disease activity.

Biomedical reports

Xiao JP,Wang DY,Wang XR,Yuan L,Hao L,Wang DG

MHCD0412 was used in Flow cytometry/Cell sorting to investigate the ratio of T helper cell 17 cells to single immunoglobulin IL-1-related receptor cluster of differentiation T cells in patients with systemic lupus erythematosus.

Mon Oct 01 00:00:00 EDT 2018

Microbiota-derived short-chain fatty acids promote Th1 cell IL-10 production to maintain intestinal homeostasis.

Nature communications

Sun M,Wu W,Chen L,Yang W,Huang X,Ma C,Chen F,Xiao Y,Zhao Y,Ma C,Yao S,Carpio VH,Dann SM,Zhao Q,Liu Z,Cong Y

MHCD0412 was used in Flow Cytometry to elucidate the various roles of gut microbiota-derived short-chain fatty acids in the maintenance of intestinal homeostasis.

Mon Sep 03 00:00:00 EDT 2018

A novel method for assaying human regulatory T cell direct suppression of B cell effector function.

Journal of immunological methods

Weingartner E,Courneya JP,Keegan A,Golding A

MHCD0412 was used in flow cytometry to develop a method to assess the capacity of human regulatory T cells to suppress IgM production from an immature human B cell line

Wed Feb 01 00:00:00 EST 2017

A novel method for assaying human regulatory T cell direct suppression of B cell effector function.

Journal of immunological methods

Weingartner E,Courneya JP,Keegan A,Golding A

MHCD0412 was used in flow cytometry to develop a method to assess the capacity of human regulatory T cells to suppress IgM production from an immature human B cell line

Wed Feb 01 00:00:00 EST 2017

Regulatory T cells and human myeloid dendritic cells promote tolerance via programmed death ligand-1.

PLoS biology

Amarnath S,Costanzo CM,Mariotti J,Ullman JL,Telford WG,Kapoor V,Riley JL,Levine BL,June CH,Fong T,Warner NL,Fowler DH

MHCD0412 was used in flow cytometry to report that human Tregs promote the generation of myeloid dendritic cells which abrogate post transplant lethality.

Tue Feb 02 00:00:00 EST 2010

Apoptosis of CD4(+)CD25(high) T cells in response to Sirolimus requires activation of T cell receptor and is modulated by IL-2.

Cancer immunology, immunotherapy : CII

Molhoek KR,McSkimming CC,Olson WC,Brautigan DL,Slingluff CL

Mon Jun 01 00:00:00 EDT 2009

生物信息学

蛋白别名: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

基因别名: CD4; CD4mut

UniProt ID: (Human) P01730

Entrez Gene ID: (Human) 920

功能

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

参与通路

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

CD4 Monoclonal Antibody (S3.5), PE-Cyanine7

产品信息

MHCD0412

流式细胞分析 (Flow)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

激发/发射光谱

形式

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

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