Invitrogen

EEF2 Recombinant Polyclonal Antibody (20HCLC)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他24个EEF2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

EEF2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

EEF2 Antibody (711842) in ICC/IF

For immunofluorescence analysis, HCT116 cells were fixed and permeabilized for detection of endogenous EEF2 using Anti- EEF2 Recombinant Rabbit Polyclonal Antibody (Product # 711842, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EEF2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S3693... View More

Product Image Gallery

EEF2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with EEF2 siRNA and decrease in signal intensity was observed in Western blot application using Anti-EEF2 Recombinant Rabbit Polyclonal Antibody (Product # 711842) {KD}

产品信息

711842

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2.5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Polyclonal

类型

Antibody

克隆号

20HCLC

抗原

Protein corresponding to human EEF2 (aa241-aa340)

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2716917

产品详细信息

This antibody is predicted to react with Monkey, Pig, Bovine, Sheep

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。