Invitrogen

FABP4 Polyclonal Antibody

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FABP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FABP4 on paraffin-embedded mouse intestine tissue. Sample was incubated with FABP4 polyclonal antibody (Product# PA5-79231).

FABP4 Antibody in Western Blot (WB) — Western blot analysis of FABP4 in, Lane 1: human RT4 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with FABP4 Polyclonal Antibody (Product # PA5-79231) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for FABP4 at approximately 15 kDa. The expected band size for FABP4 is at 15 kDa.

产品信息

PA5-79231

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the N-terminus of human FABP4 (10-40aa KLVSSENFDDYMKEVGVGFATRKVAGMAKPN).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

RRID

AB_2746347

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

FABP4 belongs to the fatty acid binding protein (FABP) family. FABPs contribute to the transport of fatty acids and other lipids in various cellular pathways. FABP4 is expressed in adipocytes, macrophages, monocyte-derived dendritic cells, and myoepithelial cells. It delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 3T3-L1 lipid-binding protein; A-FABP; adipocyte fatty acid binding protein; Adipocyte lipid binding protein; Adipocyte lipid-binding protein; adipocyte protein aP2; Adipocyte-type fatty acid-binding protein; AFABP; ALBP; epididymis secretory protein Li 104; Fatty acid binding protein 4 adipocyte; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; Fatty acid-binding protein, adipocyte; HEL S 104; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422

基因别名: 422/aP2; A-FABP; AFABP; ALBP; ALBP/Ap2; Ap2; FABP4; HEL-S-104; Lbpl

UniProt ID: (Human) P15090, (Mouse) P04117

Entrez Gene ID: (Human) 2167, (Mouse) 11770, (Rat) 79451

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