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Description: The monoclonal antibody 1H8noggin recognizes human noggin. Noggin is a secreted, glycosylated, chemokine that binds and inhibits TGFbeta (transforming growth factor beta) family members such as bone morphogenic protein-4 and -2 (BMP-4, BMP-2). The binding of noggin to BMPs prevents interaction with signal-transducing BMP receptors (BMPR), responsible for activation of VEGF and other pathways that affect vascular patterning, neural tube formation, mesodermal differentiation, and oligodendrogliogenesis. Noggin expression is restricted both temporally and by cell type, for example by chondrogenic precursor cells during limb development, ectodermal cells in the dorsal neural tube, ependymal cells adjacent to the subventricular zone in adult brain, and stromal cells underlying luminal epithelium.
Applications Reported: This 1H8noggin antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This 1H8noggin antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. The morphogenesis of organs is initiated by a downgrowth from a layer of epithelial stem cells. This process is achieved through the receipt of signals from 1) a WNT protein (WNT3A) to stabilize beta-catenin; and 2) Noggin, which is a bone morphogenetic protein inhibitor. Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have been identified, which have multiple joint fusion as their principal defect.
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