Invitrogen

VEGF Receptor 3 Monoclonal Antibody (AFL4), Biotin, eBioscience™

Product Image Gallery

VEGF Receptor 3 Antibody in Western Blot (WB) — Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Western Blot

VEGF Receptor 3 Antibody in Immunohistochemistry (IHC) — Autophagy deficiency impairs expression of lymphatic markers. a , b Representative blots for the indicated proteins in si CTRL, si ATG5 or CQ-(25 uM, 48 h) treated LEC. Densitometric quantification is indicated beneath the blots. Mean +- SD, N >= 3 biological replicates analyzed by one-way ANOVA, with Tukey's test for multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 vs si CTRL. c Representative blot for the indicated proteins in si CTRL and si ULK1 LEC. Densitometric quantification is indicated beneath the blots. Mean +- SD, N >= 3 biological replicates analyzed using unpaired Student's t test, * p < 0.05, ** p < 0.01 and *** p < 0.001. RT-qPCR analysis of si CTRL, si ATG5, or CQ-treated LEC. mRNA expression of VEGFR3, LYVE1, PROX1, and NR2F2 (relative to HPRT). Mean +- SD, N = 3 ( d , g ), N = 4 ( e ) or N = 6 ( f ) biological replicates analyzed by one-way ANOVA, with Tukey's test for multiple comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0001 vs si CTRL. h - k RT-qPCR analysis of si CTRL and si ULK1 LEC. mRNA expression of VEGFR3, LYVE1, PROX1, and NR2F2 (relative to HPRT). Mean +- SD, N = 3 ( i , j ), N = 4 ( h , k ) biological replicates analyzed by unpaired Student's t test (two-tailed), * p < 0.05, ** p < 0.01, **** p < 0.0001. l Representative immunofluorescent images of LYVE1+ lymphatic vessels (dashed lines) from corneal sections dissected from wild type (WT) and LEC-Atg5 knock out mice (LEC-Atg5 -/- ) stained for-V

VEGF Receptor 3 Antibody in Flow Cytometry (Flow) — Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Flow Cytometry

产品信息

13-5988-82

应用	建议稀释比	已发表文章
流式细胞分析 (Flow)	0.5 µg/test	查看8篇已发表文章 8篇已发表文章
免疫组化 (IHC)	-	查看7篇已发表文章 7篇已发表文章
免疫印迹 (WB)	-	查看3篇已发表文章 3篇已发表文章

产品规格
种属反应	Mouse
已发表种属	Human, Mouse
宿主/亚型	Rat / IgG2a, kappa
推荐的同型对照	Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	AFL4
偶联物	Biotin Functional Grade Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_466851

产品详细信息

Description: The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4. This 195 kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family. During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis. VEGF-C and VEGF-D bind to and activate VEGFR-3. AFL4 is an antagonist mAb.

Applications Reported: The AFL4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis.

Nature communications

Meçe O,Houbaert D,Sassano ML,Durré T,Maes H,Schaaf M,More S,Ganne M,García-Caballero M,Borri M,Verhoeven J,Agrawal M,Jacobs K,Bergers G,Blacher S,Ghesquière B,Dewerchin M,Swinnen JV,Vinckier S,Soengas MS,Carmeliet P,Noël A,Agostinis P

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Immunohistochemistry

Thu May 19 00:00:00 EDT 2022

Peptides Targeting Fms-Related Tyrosine Kinase-4 Activate the Function of Natural Killer Cells in Acute Myeloid Leukemia.

International journal of stem cells

Park S,Kim HJ,Hwang HS,Han AR

13-5988-82 was used in Flow Cytometry to show that there was a trend toward a reduction in leukemic blasts in the BM, spleen, and liver from the AML mice, when we compared the effects of combinational treatments.

Tue Nov 30 00:00:00 EST 2021

Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration.

Cell reports

Gadomski S,Singh SK,Singh S,Sarkar T,Klarmann KD,Berenschot M,Seaman S,Jakubison B,Gudmundsson KO,Lockett S,Keller JR

13-5988 was used in Flow cytometry/Cell sorting to investigate the effects of loss of ld genes on adult endothelial cell.

Tue Apr 28 00:00:00 EDT 2020

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

13-5988 was used in Flow cytometry/Cell sorting to identify that lung endothelium and proangiogenic haematopoietic progenitor cells in the airway sense allergen and elicit an angiogenic response that is central to the innate non-immune origins of Th2 inflammation.

Mon Jul 02 00:00:00 EDT 2018

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

Mon Jul 02 00:00:00 EDT 2018

G-Protein-Coupled Receptor-2-Interacting Protein-1 Controls Stalk Cell Fate by Inhibiting Delta-like 4-Notch1 Signaling.

Cell reports

Majumder S,Zhu G,Xu X,Senchanthisai S,Jiang D,Liu H,Xue C,Wang X,Coia H,Cui Z,Smolock EM,Libby RT,Berk BC,Pang J

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Western Blot

Tue Dec 06 00:00:00 EST 2016

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Thu Dec 01 00:00:00 EST 2016

VEGF-D promotes pulmonary oedema in hyperoxic acute lung injury.

The Journal of pathology

Sato T,Paquet-Fifield S,Harris NC,Roufail S,Turner DJ,Yuan Y,Zhang YF,Fox SB,Hibbs ML,Wilkinson-Berka JL,Williams RA,Stacker SA,Sly PD,Achen MG

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Immunohistochemistry

Wed Jun 01 00:00:00 EDT 2016

A Selenium Containing Inhibitor for the Treatment of Hepatocellular Cancer.

Pharmaceuticals (Basel, Switzerland)

Tagaram HR,Desai D,Li G,Liu D,Rountree CB,Gowda K,Berg A,Amin S,Staveley-O'Carroll KF,Kimchi ET

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Immunohistochemistry

Thu Mar 24 00:00:00 EDT 2016

Vascular endothelial growth factor c/vascular endothelial growth factor receptor 3 signaling regulates chemokine gradients and lymphocyte migration from tissues to lymphatics.

Transplantation

Iwami D,Brinkman CC,Bromberg JS

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

ID3 contributes to the acquisition of molecular stem cell-like signature in microvascular endothelial cells: its implication for understanding microvascular diseases.

Microvascular research

Das JK,Voelkel NF,Felty Q

13-5988 was used in Flow cytometry/Cell sorting to show that ID3 overexpressing cells supported the formation of a 3-D microvascular lesion co-cultured with smooth muscle cells.

Sun Mar 01 00:00:00 EST 2015

Arteriolar niches maintain haematopoietic stem cell quiescence.

Nature

Kunisaki Y,Bruns I,Scheiermann C,Ahmed J,Pinho S,Zhang D,Mizoguchi T,Wei Q,Lucas D,Ito K,Mar JC,Bergman A,Frenette PS

13-5988 was used in Flow cytometry/Cell sorting to investigate the spatial localisation of quiescent haematopoietic stem cells in bone marrow, showing that arteriolar niches maintain quiescence.

Thu Oct 31 00:00:00 EDT 2013

Fbxw7 controls angiogenesis by regulating endothelial Notch activity.

PloS one

Izumi N,Helker C,Ehling M,Behrens A,Herzog W,Adams RH

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Immunofluorescence

Tue Apr 02 00:00:00 EDT 2013

Kidins220/ARMS mediates the integration of the neurotrophin and VEGF pathways in the vascular and nervous systems.

Cell death and differentiation

Cesca F,Yabe A,Spencer-Dene B,Scholz-Starke J,Medrihan L,Maden CH,Gerhardt H,Orriss IR,Baldelli P,Al-Qatari M,Koltzenburg M,Adams RH,Benfenati F,Schiavo G

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Western Blot

Wed Feb 01 00:00:00 EST 2012

Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature.

BMC developmental biology

Kim H,Nguyen VP,Petrova TV,Cruz M,Alitalo K,Dumont DJ

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Immunohistochemistry

Mon Jun 28 00:00:00 EDT 2010

Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature.

BMC developmental biology

Kim H,Nguyen VP,Petrova TV,Cruz M,Alitalo K,Dumont DJ

Published figure using VEGF Receptor 3 monoclonal antibody (Product # 13-5988-82) in Immunohistochemistry

Mon Jun 28 00:00:00 EDT 2010

Lack of lymphatic vessel phenotype in LYVE-1/CD44 double knockout mice.

Journal of cellular physiology

Luong MX,Tam J,Lin Q,Hagendoorn J,Moore KJ,Padera TP,Seed B,Fukumura D,Kucherlapati R,Jain RK

13-5988-82 was used in Immunohistochemistry to study whether other hyaluronan receptors, such as CD44, may compensate for LYVE-1.

Fri May 01 00:00:00 EDT 2009

Postnatal bone marrow stromal cells elicit a potent VEGF-dependent neoangiogenic response in vivo.

Gene therapy

Al-Khaldi A,Eliopoulos N,Martineau D,Lejeune L,Lachapelle K,Galipeau J

13-5988 was used in Flow cytometry/Cell sorting to determine the functional neoangiogenic potential of bone marrow stromal cells in vivo.

Tue Apr 01 00:00:00 EST 2003

生物信息学

蛋白别名: chylous ascites; FLT-4; Fms-like tyrosine kinase 4; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; Tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR-3

基因别名: AI323512; Chy; Flt-4; Flt4; VEGFR-3; VEGFR3

UniProt ID: (Mouse) P35917

Entrez Gene ID: (Mouse) 14257

分子生物学功能: transmembrane signal receptor

功能

参与通路

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding protein phosphatase binding

VEGF Receptor 3 Monoclonal Antibody (AFL4), Biotin, eBioscience™

产品信息

13-5988-82

流式细胞分析 (Flow)

免疫组化 (IHC)

免疫印迹 (WB)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

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