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Invitrogen
| 应用 | 建议稀释比 |
|---|---|
免疫印迹 (WB) |
1:5,000-1:20,000 |
免疫组化 (IHC) |
1:50-1:500 |
免疫细胞化学 (ICC/IF) |
1:50-1:500 |
流式细胞分析 (Flow) |
1:50 - 1:200 |
免疫沉淀 (IP) |
Assay-dependent |
| 产品规格 | |
|---|---|
种属反应 |
Rabbit |
宿主/亚型 |
Goat / IgG |
分类 |
Polyclonal |
类型 |
Secondary Antibody |
抗原 |
Rabbit IgG-heavy and light chain |
偶联物 |
DyLight™ 550
|
形式 |
Liquid |
浓度 |
0.5 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
PBS with 0.2% BSA |
内含物 |
0.09% sodium azide |
保存条件 |
4° C |
RRID |
AB_2556613 |
靶标 |
IgG |
交叉吸附 |
Against bovine, chicken, horse, human, mouse, pig and rat |
抗体形式 |
Whole Antibody |
This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, horse, human, mouse, pig and rat.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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