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The Mouse BLC/CXCL13 ELISA quantitates Ms BLC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms BLC.
原理方法
The Mouse BLC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in the secondary lymphoid organs. It preferentially promotes the migration of B lymphocytes (both B-1 and B-2) to the follicles of the spleen, lymph nodes, and Peyer's patches. It is produced by macrophages, and in mouse the highest constitutive producers include large peritoneal macrophages, Kuppfer cells and lung macrophages (both CD11c+ and CD11c-). The cellular ligand for CXCL13 is CXCR5 also known as BLR-1 (Burkitt’s Lymphoma Receptor 1). CXCR5 is expressed on B cells, Tfh cells and, to a lower degree, some memory T cell populations. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL). CXCL13 is also a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : 4631412M08Rik, Angie, ANGIE2, BCA-1, BLC, BLR1L, Cxcl13, Scyb13
基因ID : (Mouse) 55985
基因符号 : Cxcl13
蛋白别名 : B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, C-X-C motif chemokine 13, small inducible cytokine subfamily B (Cys-X-Cys), member 13
UniProt ID (Mouse) O55038