Search Thermo Fisher Scientific
The Mouse CD40 Ligand (Ms CD40L) ELISA quantitates Ms CD40L in mouse serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms CD40L.
原理方法
The Mouse CD40L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : CD154, CD40-L, Cd40l, Cd40lg, gp39, HIGM1, IGM, IMD3, Ly-62, Ly62, T-BAM, Tnfsf5, TRAP
基因ID : (Mouse) 21947
基因符号 : Cd40lg
蛋白别名 : CD154, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5
UniProt ID (Mouse) P27548