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The Mouse Th1/Th2 Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse Th1/Th2 proteins (Interferon gamma, IL-2, IL-4 and IL-10). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
T helper type 1 (Th1) cells and T helper type 2 (Th2) cells are both a lineage of CD4 effector T cells. Th1 and Th2 are involved in promoting cell-mediated immunity. Th1 cells secrete IFN-gamma, IL-2, IL-10, and TNF-alpha/beta, which are required for host defense against intracellular viral and bacterial pathogens. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL-17E/IL-15, which are required for humoral immunity and play an important role in coordinating the immune response to larger extracellular pathogens.
仅用于科研。不用于诊断过程。未经明确授权不得转售。