Search Thermo Fisher Scientific
蛋白质凝胶1D电泳支持
有关常见问题的解决方案，请参阅“入门”和“故障排除”部分。有关各种产品相关的支持资源，请参阅“指南和工具”部分。
故障排除
- My protein bands in the outer lanes of the gel show a “smiling” effect. Can you please help me troubleshoot?
- I am getting wavy dye fronts while using the Bolt Bis-Tris Plus gels with the Bolt tank. Why is this?
- When I tried to remove the comb from my Bolt Bis-Tris Plus gel, I noticed the presence of extra acrylamide. What is the purpose?
- I ran my protein samples on a NuPAGE Bis-Tris gel, and I see a bubble in the center of the gel with significant band distortion particularly in the center lanes. What is the reason?
- I ran my protein samples on a Tris-Glycine gel and the samples stopped running in the bottom portion of the gel whereas the marker ran fine. Why did this happen?
指南及工具
General
- Overview: Protein Gel Electrophoresis
- Overview: Novex™ Precast Midi Gels
- Overview: Protein Separation by Protein Electrophoresis
- Learning Center: Protein Biology
- Protein Biology Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Guide: Protein Analysis Handbook
- Selection: Find the Right Protein Gel for Your Experiments
- Selection: Protein Mini Gel Selection Guide
- Selection: Find our Equivalent to Competitor Gels
- Technical Note: Protein Separation Myths
- Technical Publication: Models of Protein Modification in Tris-Glycine and Neutral pH Bis-Tris Gels during Electrophoresis: Effect of Gel pH
- Video: Protein Analysis Tips and Tricks
- Video: Novex™ Precast Protein Gels – Beauty of a Straight Line
- Video: Novex™ Gel Cassettes – Easy, Simple Solutions that Make a Big Difference
- Video: Separate Proteins by SDS-PAGE for Western Blot
Novex™ Tris-Glycine Gels
- Overview: Novex™ Tris-Glycine Gels
- Gel Migration Chart: Novex™ Tris-Glycine Gels (for optimal results, protein bands should migrate within the yellow shaded areas)
- Selection: Choose the Right Novex™ Tris-Glycine Gel
NuPAGE™ Bis-Tris and NuPAGE™ Tris-Acetate Gels
- Overview: Novex™ NuPAGE™ SDS-PAGE Gel System
- Gel Migration Chart: NuPAGE™ Bis-Tris Gels (for optimal results, protein bands should migrate within the yellow shaded areas)
- Gel Migration Chart: NuPAGE™ Tris-Acetate Gels (for optimal results, protein bands should migrate within the yellow shaded areas)
- Selection: Choose the Right NuPAGE™ Bis-Tris Gel
- Selection: Choose the Right NuPAGE™ Tris-Acetate Gel
- Video: NuPAGE™ Novex™ Gel System
- Video: Meet The Inventor: NuPAGE™ System
Bolt™ Bis-Tris Plus Gels
- Overview: Bolt™ Bis-Tris Plus Gels
- Overview: Resources for Upgrading the Bolt™ Mini Gel Tank
- Gel Migration Chart: Bolt™ Bis-Tris Plus gels (for optimal results, protein bands should migrate within the yellow shaded areas)
- Selection: Bolt™ Gel Selection Guide
- Video: Operating the Mini Gel Tank
- Video: Replacing Cam Handles on Bolt™ Mini Gel Tank
Precise™ Protein Gels
- Overview: Precise™ Protein Gels
- Choose the Right Protein Mini Gel
- Choose the Right Novex™ Tris-Glycine Gel
- Choose the Right NuPAGE™ Bis-Tris Gel
- Choose the Right NuPAGE™ Tris-Acetate Gel
- Choose the Right BOLT™ Bis-Tris Plus Gel
- Choose the Right Precast Midi Gel
- NuPAGE ™ Gel Conversion Guide: Easy and simple conversion from either a Tris-Glycine or Tricine gel to the appropriate NuPAGE™ gel and corresponding optimized buffer.
- Using gels from another vendor? Find the right equivalent gel from our company
- DailyCalcs Science Calculator: An app designed to calculate molarity, dilution, molecular weight, and more. Also includes a transfection calculator you can use to simplify transfection experimental setup.
定制服务
GeneArt细胞系及蛋白服务
GeneArt 细胞系及蛋白服务是哺乳动物细胞蛋白表达的一条龙服务包。我们可以帮助改进工作流程中的每一步骤，包括基因设计、合成、克隆及蛋白制备、甚至是稳定细胞系构建等。同样，我们也可根据你的需求改进流程中的某些步骤。
蛋白表达及制备服务
利用我们丰富的表达载体和宿主系统组合，达到所期望的蛋白得率。我们不仅提供杆状病毒和哺乳动物宿主系统蛋白表达和制备服务，而且还提供蛋白纯化和分析服务。
ProtoArray™服务
我们经验丰富的科学家可为您优化实验设计，使您获得最理想的结果。
仅限研究用途，不可用于诊断操作。