Low oxygen tension and autologous plasma enhance T-cell proliferation and CD49d expression density in serum-free media.
Authors Haddad H; Papoutsakis E T;
JournalCytotherapy
PubMed ID11953028
'Background As cellular immunotherapy with ex vivo expanded cells becomes more widely used to treat a variety of illnesses, optimization of culture parameters, to maximize cell production and function, is essential for continued success. The effects of reduced oxygen tension and autologous plasma on T-cell expansion, receptor expression, apoptosis, and ... More
Effects of interleukin 4 upon human tumoricidal cells obtained from patients bearing solid tumors.
Authors Jadus M R; Good R W; Crumpacker D B; Yannelli J R;
JournalJ Leukoc Biol
PubMed ID1991997
'The use of human interleukin 4 (IL4) in the generation of tumoricidal effector cells derived from cancer patients undergoing either interleukin 2/lymphokine activated killer cells (IL2/LAK) therapy or tumor derived activated cell (TDAC) therapy was examined in the present study. Human IL4 alone did not generate LAK cells from patients ... More
Dominant negative effectors of heparin affin regulatory peptide (HARP) angiogenic and transforming activities.
'Heparin affin regulatory peptide (HARP) is an heparin-binding growth factor, highly expressed in several primary human tumors and considered as a rate-limiting angiogenic factor in tumor growth, invasion, and metastasis. Implication of this protein in carcinogenesis is linked to its mitogenic, angiogenic, and transforming activities. Recently, we have demonstrated that ... More
T(84) epithelial cells respond to 5-hydroxytryptamine when grown in serum-free media.
Authors Burleigh D; Fernandes K; Perrett D;
JournalEur J Pharmacol
PubMed ID10708712
The aim of this study was to establish the cause of insensitivity of T(84) human colonic epithelial cells to 5-hydroxytryptamine (5-HT). Monolayers of T(84) cells were placed in modified Ussing chambers for measurement of short-circuit current, an index of secretion. When grown in serum-supplemented media, T(84) cells gave secretory responses ... More
Generation of human autologous melanoma-specific cytotoxic T cells from tumor-involved lymph nodes.
Authors Maeda K; Lafreniere R; McKinnon J G; Jerry L M;
JournalMol Biother
PubMed ID1910625
Cytotoxic T lymphocytes (CTL) specific for autologous human melanoma have been successfully generated in vitro from tumor bearing lymph nodes without any stimulation by the autologous tumor. Tumor-involved lymph node cells (LNC) were cultured in serum free medium (AIM-V) containing 1,000 U/ml of recombinant interleukin-2. The best expansion and specific ... More
[Function of dendritic cell in chronic hepatitis C patients]
Authors Li Xiaoguang; Lu Shulan; Wang Guiqiang; Yue Bei; Wang Zhihong;
JournalZhonghua Nei Ke Za Zhi
PubMed ID12133427
OBJECTIVE: To explore the relation between dendritic cell (DC) and the persistence of HCV in chronic hepatitis C. METHODS: The mononuclear cells were separated from the periphral blood and cultured with the AIM-V serum-free culture to induce DC. The shape and the ultrastructure of DC, the phenotype of DC, the ... More
An improved procedure for the development of human mast cells from dispersed fetal liver cells in serum-free culture medium.
Authors Kambe N; Kambe M; Chang H W; Matsui A; Min H K; Hussein M; Oskerizian C A; Kochan J; Irani A A; Schwartz L B;
JournalJ Immunol Methods
PubMed ID10854605
The in vitro development of human mast cells from fetal liver cells with recombinant human stem cell factor in serum-containing RPMI was compared to that in AIM-V media with and without serum. Compared to serum-containing media, AIM-V medium caused mast cells to develop earlier and in greater numbers. By 2 ... More
Culture of human tumor infiltrating lymphocytes in hollow fiber bioreactors.
Authors Knazek R A; Wu Y W; Aebersold P M; Rosenberg S A;
JournalJ Immunol Methods
PubMed ID2319140
Human tumor infiltrating lymphocytes (TIL) from metastatic melanoma of six patients were grown using a new hollow fiber bioreactor system. After inoculating 0.35-10 X 10(8) TIL into the extra-fiber space (EFS), each Cellmax bioreactor was perfused with AIM-V medium, supplemented with rIL-2. The cells subsequently expanded 124-1170-fold to yield 1.5-5.4 ... More
Ex vivo expansion of functional T lymphocytes from HIV-infected individuals.
Authors Trickett Annette E; Kwan Yiu Lam; Cameron Barbara; Dwyer John M;
JournalJ Immunol Methods
PubMed ID11983220
This study was designed to define the conditions for expansion of functional T lymphocytes from human immunodeficiency virus (HIV)-infected subjects, with the ultimate goal of using these cells for immunotherapy. The most appropriate culture conditions for good T cell proliferation included stimulation with anti-CD3 and anti-CD28 coated microspheres, and propagation ... More
Differential association of HLA-B*2705 and B*2709 to ankylosing spondylitis correlates with limited peptide subsets but not with altered cell surface stability.
Authors Ramos Manuel; Paradela Alberto; Vazquez Miriam; Marina Anabel; Vazquez Jesus; Lopez de Castro Jose A;
JournalJ Biol Chem
PubMed ID12042320
In contrast to HLA-B*2705, B*2709 is weakly or not associated to ankylosing spondylitis. Both allotypes differ by a single D116H change. We compared the B*2705- and B*2709-bound peptide repertoires by mass spectrometry to quantify the effect of B*2709 polymorphism on peptide specificity. In addition, shared and differentially bound ligands were ... More
Enhancement of lymphocyte proliferation assays by use of serum-free medium.
Authors Kaldjian E P; Chen G H; Cease K B;
JournalJ Immunol Methods
PubMed ID1532181
Fetal bovine serum (FBS)-supplemented cell culture medium has been the standard medium used in assays of murine lymphocyte proliferation. While FBS allows cell growth and viability, it requires screening and contains uncharacterized elements which may yield inconsistent results from batch to batch. It also may include growth factors and immunologically ... More
Adenoviral transduction of human 'clinical grade' immature dendritic cells enhances costimulatory molecule expression and T-cell stimulatory capacity.
The therapeutic use of dendritic cells (DC) in antigen-specific anti-tumor vaccines, requires sufficient numbers of functional DC, the preparation of which should comply with the code of Good Manufacturing Practice. In addition, the expression of tumor specific antigen should be possible in these DC. As a preclinical step, the method ... More
Lymphokine-activated killer cells: culture conditions for the generation of maximal in vitro cytotoxicity in cells from normal donors.
Authors Beckner S K; Maluish A E; Longo D L;
JournalCancer Res
PubMed ID3498534
The current method for generating lymphokine-activated killer (LAK) cells for use in human clinical trials is both labor intensive and expensive. Therefore, we altered cell culture conditions to determine whether LAK cells with enhanced lytic activity could be generated. Culture of normal human peripheral blood leukocytes for 7 days generated ... More
IL-6 attenuates apoptosis, while neither IL-6 nor IL-10 affect the numbers or protease phenotype of fetal liver-derived human mast cells.
Authors Kambe M; Kambe N; Oskeritzian C A; Schechter N; Schwartz L B;
JournalClin Exp Allergy
PubMed ID11467999
BACKGROUND: The combination of recombinant human stem cell factor (rhSCF), rh interleukin (IL)-6 and rhIL-10 was reported to be optimal for mast cell development from cord blood progenitors and to induce chymase expression in all such mast cells earlier in their development than tryptase. OBJECTIVE: The effects of rhIL-6 and ... More
Messenger RNA electroporation of human monocytes, followed by rapid in vitro differentiation, leads to highly stimulatory antigen-loaded mature dendritic cells.
Authors Ponsaerts Peter; Van Den Bosch Glenn; Cools Nathalie; Van Driessche Ann; Nijs Griet; Lenjou Marc; Lardon Filip; Van Broeckhoven Christine; Van Bockstaele Dirk R; Berneman Zwi N; Van Tendeloo Viggo F I;
JournalJ Immunol
PubMed ID12165485
Dendritic cells (DC) are professional Ag-capturing and -presenting cells of the immune system. Because of their exceptional capability of activating tumor-specific T cells, cancer vaccination research is now shifting toward the formulation of a clinical human DC vaccine. We developed a short term and serum-free culture protocol for rapid generation ... More
Combination of lymphokine-activated killer cells and interleukin-2 in treating metastatic renal cell carcinoma.
OBJECTIVE: To study the properties of lymphokine-activated killer (LAK) cells and the effect of immunotherapy with a combination of autologous LAK cells and interleukin-2 (IL-2) [LAK therapy] in 10 patients with metastatic renal cell carcinoma (RCC). MATERIALS AND METHODS: The LAK cells were generated from peripheral blood lymphocytes (PBL) by ... More
[Functions of cultured dendritic cells from patients with chronic hepatitis Beta decreased]
Authors Li Ruobing; Chen Hongsong; Fei Ran; Et Al;
JournalZhonghua Yi Xue Za Zhi
PubMed ID12126512
OBJECTIVE: To compare the phenotype and function of dendritic cells (DCs) of patients with chronic hepatitis Beta with those of DCs from healthy persons. METHOD: Peripheral blood monocytes (PBMCs) were isolated from 10 patients with chronic hepatitis Beta and 10 healthy blood donors. DCs were generated by culturing PBMCs in ... More
Immunotherapy of murine hepatic metastases with lymphokine-activated killer cells expanded in serum-free media and recombinant interleukin 2.
Authors Lafreniere R; Borkenhagen K; Bryant L D; Huchcroft S;
JournalCancer Res
PubMed ID2565160
It has been shown that the systemic administration of lymphokine-activated killer (LAK) cells with recombinant interleukin 2 (RIL-2) is effective in reducing the number of established pulmonary and hepatic metastases in murine models. Similarly, this modality of therapy has been proven effective against certain selected human tumors as well. In ... More
Large-scale expansion in interleukin-2 of tumor-infiltrating lymphocytes from patients with ovarian carcinoma for adoptive immunotherapy.
Authors Freedman R S; Tomasovic B; Templin S; Atkinson E N; Kudelka A; Edwards C L; Platsoucas C D;
JournalJ Immunol Methods
PubMed ID8308273
Tumor infiltrating lymphocytes (TIL) from malignant ascites or solid tumor specimens obtained from patients with ovarian carcinoma were expanded to large numbers in vitro (10(10)-10(11)) by a four-step method using AIM V medium and low concentrations of recombinant interleukin-2 (rIL-2). The expansion procedure employed 24-well culture plates, T-flasks, polyolefin gas-permeable ... More
Lymphoproliferative responses in cord blood and at one year: no evidence for the effect of in utero exposure to dust mite allergens.
Authors Smillie F I; Elderfield A J; Patel F; Cain G; Tavenier G; Brutsche M; Craven M; Custovic A; Woodcock A;
JournalClin Exp Allergy
PubMed ID11529888
BACKGROUND: Maternal allergen exposure beyond the 22nd week of pregnancy may be important in foetal T cell priming. Allergen-specific cord blood mononuclear cell (CBMC) immunoproliferative responses without corresponding bacterial antigen responses (tetanus toxoid), have been suggested as evidence of in utero sensitization. OBJECTIVES: To investigate the relationship between lymphoproliferative responses ... More
Long-term cultivation of functional human macrophages in Teflon dishes with serum-free media.
Authors Helinski E H; Bielat K L; Ovak G M; Pauly J L;
JournalJ Leukoc Biol
PubMed ID3404068
Reported herein are the results of studies demonstrating the utility of a chemically defined, serum-free medium designated as AIM-V (GIBCO) for the long-term (greater than 2 weeks) cultivation of functionally-defined human macrophages. The AIM-V medium is a mixture of HEPES-buffered Dulbecco's Modified Eagle Medium and Ham's Nutrient Mixture F12 that ... More
Malignant effusions and immunogenic tumour-derived exosomes.
BACKGROUND: Exosomes derived from tumours are small vesicles released in vitro by tumour cell lines in culture supernatants. To assess the role of these exosomes in vivo, we examined malignant effusions for their presence. We also investigated whether these exosomes could induce production of tumour-specific T cells when pulsed with ... More
Induction of MHC class II on human polymorphonuclear neutrophils by granulocyte/macrophage colony-stimulating factor, IFN-gamma, and IL-3.
Authors Gosselin E J; Wardwell K; Rigby W F; Guyre P M;
JournalJ Immunol
PubMed ID8335942
Polymorphonuclear neutrophils (PMN) have long been thought to be short-lived, terminally differentiated cells incapable of synthesizing significant levels of protein, with their primary function being phagocytosis and the release of cytotoxic compounds. More recently, it has been demonstrated that PMN can produce a number of functionally diverse substances, including IL-1, ... More
Tumor-infiltrating lymphocytes from nonrenal urological malignancies.
Authors Haas G P; Solomon D; Rosenberg S A;
JournalCancer Immunol Immunother
PubMed ID2105845
Tumor-infiltrating lymphocytes (TIL) were isolated from 15 of 20 surgical specimens of transitional cell carcinoma of the urinary bladder, prostate cancer, testicular cancer, Wilms tumor and adrenal cancer. Expansion was carried out in four different culture conditions, each containing 1000 U/ml interleukin-2: RPMI medium with or without 20% (by volume) ... More
In vitro production of dendritic cells from human blood monocytes for therapeutic use.
Dendritic cells (DC) are professional antigen-presenting cells that are promising adjuvants for clinical immunotherapy. Methods to generate in vitro large numbers of functional human DC using either peripheral blood monocytes or CD34(+) pluripotent hematopoietic progenitor cells have been now developed. For this purpose, their in vitro production for further clinical ... More
Immunity to malaria after administration of ultra-low doses of red cells infected with Plasmodium falciparum.
Authors Pombo David; Lawrence Gregor; Hirunpetcharat Chakrit; Rzepczyk Christine; Bryden Michelle; Cloonan Nicole; Anderson Karen; Mahakunkijcharoen Yuvadee; Martin Laura; Wilson Danny; Elliott Salenna; Elliott Suzanne; Eisen Damon; Weinberg J; Saul Allan; Good Michael;
JournalLancet
PubMed ID12241933
Background The ability of T cells, acting independently of antibodies, to control malaria parasite growth in people has not been defined. If such was shown to be effective, an additional vaccine strategy could be pursued. Our aim was to ascertain whether or not development of cell-mediated immunity to Plasmodium falciparum ... More
Study of tumor-infiltrating lymphocytes for adoptive therapy of renal cell carcinoma (RCC) and metastatic melanoma: sequential proliferation of cytotoxic natural killer and noncytotoxic T cells in RCC.
Authors Hayakawa K; Salmeron M A; Parkinson D R; Markowitz A B; von Eschenbach A C; Legha S S; Balch C M; Ross M I; Augustus L B; Itoh K;
JournalJ Immunother
PubMed ID1790139
We investigated the immunological properties of interleukin-2 (IL-2)-activated tumor-infiltrating lymphocytes (TIL), which were used for adoptive therapy of renal cell carcinoma (RCC) (seven patients) by focusing on natural killer (NK) cells, and metastatic melanoma (six patients) by focusing on cytotoxic T lymphocytes. TIL from five of seven cases proliferated well ... More