Ion AmpliSeq™ HD Library Kit with HD Enhancer - FAQs

查看更多产品信息 Ion AmpliSeq™ HD Library Kit with HD Enhancer - FAQs (A57283)

9 个常见问题解答

How long can 10X DNA working panel FWD and REV subpools be stored?

10X DNA working panel FWD and REV subpools can be stored at 4 degrees C for one week. For longer term storage, aliquot and store subpools at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Have there been any recent changes to the protocol for Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?

There have been some recent changes to the protocol. Please refer to the latest user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf) for full details.

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When should I use CRC versus HD Enhancer for Ion AmpliSeq HD library preparation?

CRC is a component of the Ion AmpliSeq HD Library Kit (Cat. No. A37694). CRC improves target amplification reactions for challenging panels that produce excessive amounts of primer dimer or panels that have ⋝500 primer pairs. We recommend using CRC with all panel types. HD Enhancer is a component of the Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) and is also available separately as Cat. No. A53690. HD Enhancer is used for improved library quality and molecular coverage results. Guidelines for using HD Enhancer or CRC for Ion AmpliSeq HD library preparation are outlined in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Which barcodes are compatible with Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?

We recommend using the Ion AmpliSeq HD Dual Barcode Kit 1-24 (Cat. No. A37695) with Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283). The libraries are dual barcoded at the 5' and 3' ends and this enables the multiplexing of up to 24 samples in a single chip. Using these dual barcodes also reduces the risk of barcode cross-contamination.

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What are the shipping and storage conditions for Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?

Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) is shipped on dry ice and we recommend storing it at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I purchase the HD Enhancer separately?

Yes, the HD Enhancer, Cat. No. A53690 can be ordered separately by contacting Customer Service.

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What are the advantages of using the HD Enhancer, Cat. No. A53690?

The HD Enhancer improves library quality and molecular coverage results. It also improves performance by reducing primer dimers and improves amplicon performance especially GC amplicon molecular coverage.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the components of Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?

Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) has 2 subcomponents - Ion AmpliSeq HD Library Kit (Cat. No. A37694) and HD Enhancer (Cat. No. A53690).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reactions can I run using Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?

Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) is sufficient to run 24 reactions.<

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.