含 HD 增强剂的 Ion AmpliSeq™ HD 文库试剂盒
含 HD 增强剂的 Ion AmpliSeq™ HD 文库试剂盒
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含 HD 增强剂的 Ion AmpliSeq™ HD 文库试剂盒

Ion AmpliSeq HD 文库试剂盒(含 HD 增强剂),设计用于手动产生独特分子标签 (UMT) 靶向的扩增子文库,作为了解更多信息
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货号Quantity
A57283
货号 A57283
价格(CNY)
-
Ion AmpliSeq HD 文库试剂盒(含 HD 增强剂),设计用于手动产生独特分子标签 (UMT) 靶向的扩增子文库,作为 Ion AmpliSeq HD 测序工作流程的一部分。本产品包括 Ion AmpliSeq HD 文库试剂盒(货号 A37694)和 HD 增强剂试剂盒,用于改善文库质量和分子覆盖结果。本产品与使用 Ion AmpliSeq Designer 工具 (www.ampliseq.cn) 创建的定制检测组合兼容,并使用 Ion GeneStudio S5 系列仪器进行测序。

Ion AmpliSeq HD 技术可实现从12通路到5000通路(在1池检测组合中为 1 x 5000-通路,或在2池检测组合中为 2 x 2500-通路)的可扩展聚合酶链反应 (PCR),适用于 UMT 靶向测序,检测水平低至 0.1% 变异频率,具体取决于样本类型、样本输入量和读取覆盖深度。

主要产品特点包括:
• 定制设计创建
• 超高灵敏度检测
• 多样本通过双条形码可同时检测
• 双向测序
• 文库制备性能得到改善

定制设计创建
Ion AmpliSeq Designer 工具现在可用于创建与 Ion AmpliSeq HD 技术配合使用的定制设计。设计可定制,允许用户灵活创建区域、热点或融合设计。这些是一池或两池设计,推荐用于从 cfDNA、cfRNA、cfTNA、FFPE DNA、FFPE RNA、gDNA 或总 RNA 中获得的样品。

超高灵敏度检测
Ion AmpliSeq HD 技术能够进行低至 0.1% 的超高水平检测。对于具有已知变体的 cfDNA、cfTNA 和 gDNA 对照,已经在足够的读取覆盖深度下使用 ≥ 20 ng 的起始量观察到这些检测水平。其他类型样品(如 FFPE)的检测水平会有所不同。

双条形码样品倍增
含有 HD 增强剂的 Ion AmpliSeq HD 文库试剂盒,包括使用 Ion AmpliSeq HD 引物生成扩增子的试剂,用于制备测序文库。使用 Ion AmpliSeq HD 双条形码试剂盒 1-24,在5’和3’端对文库进行双条形编码,该试剂盒可在单个芯片中同时检测多达24份样品。

双向测序
Ion AmpliSeq HD 文库试剂盒还提供来自两条链的测序信息。这样可以更好地纠正错误,并提高您的序列数据质量的可信度。

文库制备性能得到改善
该试剂盒中包含的新型 HD 增强剂组分,与更新的 Ion AmpliSeq HD 文库制备方案相关,通过在文库制备过程中减少引物二聚体形成并提高扩增子(特别是高 GC 扩增子分子覆盖度)性能,改善了产品性能。

仅供科研使用。不可用于诊断程序。
规格
检测方法分子标记,UMT
适用于(应用)测序
适用于(设备)Ion GeneStudio™ S5 系列测序仪
靶向测序库
反应次数24 次反应
核酸类型cfDNA、cfRNA、CfTNA、FFPE
产品线AmpliSeq
产品类型HD 文库试剂盒
样品类型来自血浆或 FFPE 的 DNA/RNA/TNA
运输条件干冰
工作流程步骤文库生成
产品规格试剂盒
测序类型新一代测序
Unit SizeEach
内容与储存
保存在 -5°C 到 -30°C。

常见问题解答 (FAQ)

How long can 10X DNA working panel FWD and REV subpools be stored?

10X DNA working panel FWD and REV subpools can be stored at 4 degrees C for one week. For longer term storage, aliquot and store subpools at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Have there been any recent changes to the protocol for Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?

There have been some recent changes to the protocol. Please refer to the latest user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf) for full details.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

When should I use CRC versus HD Enhancer for Ion AmpliSeq HD library preparation?

CRC is a component of the Ion AmpliSeq HD Library Kit (Cat. No. A37694). CRC improves target amplification reactions for challenging panels that produce excessive amounts of primer dimer or panels that have ⋝500 primer pairs. We recommend using CRC with all panel types. HD Enhancer is a component of the Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) and is also available separately as Cat. No. A53690. HD Enhancer is used for improved library quality and molecular coverage results. Guidelines for using HD Enhancer or CRC for Ion AmpliSeq HD library preparation are outlined in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Which barcodes are compatible with Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?

We recommend using the Ion AmpliSeq HD Dual Barcode Kit 1-24 (Cat. No. A37695) with Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283). The libraries are dual barcoded at the 5' and 3' ends and this enables the multiplexing of up to 24 samples in a single chip. Using these dual barcodes also reduces the risk of barcode cross-contamination.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the shipping and storage conditions for Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283)?

Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) is shipped on dry ice and we recommend storing it at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.